The influence of hydroxypropyl-β-cyclodextrin on the enantioselective hydrolysis of 2-amino phenylpropionitrile catalyzed by recombinant nitrilase  

作　　者：Ming-Yang Li Xue-Dong Wang 

机构地区：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,130 Meilong Road,Shanghai 200237,People’s Republic of China

出　　处：《Bioresources and Bioprocessing》2014年第1期209-214,共6页生物资源与生物加工(英文)

摘　　要：Background:Hydrolysis of 2-amino phenylpropionitrile by nitrilase is a fundamental biochemical reaction that produces chiral phenylalanine.For practical application of this biochemical reaction,researchers have attempted to improve enzyme enantioselectivity and the reaction rate.Results:The substrate concentration was increased from 100 to 200 mM without substrate inhibition because of the formation of a substrate-hydroxypropyl-β-cyclodextrin(HP-β-CD)complex.Meanwhile,the activity of recombinant nitrilase increased 2.5 times because the addition of HP-β-CD solubilized hydrophobic substrates in the aqueous system.Furthermore,the formation of the substrate-HP-β-CD inclusion improved the enantioselectivity of the enzymatic reaction toward producing L-phenylalanine(L-Phe).The enantiomeric excess(e.e.)value of L-Phe increased from 65%to 83%when the conversion rate reached 50%.Conclusions:The recombinant nitrilase enantioselectively hydrolyzed 2-amino phenylpropionitrile to produce L-Phe.The addition of HP-β-CD to the reaction system enhanced the solubility and bioavailability of hydrophobic substrates as well as the enantioselectivity.The results showed that this additive has potential advantages in biochemical reactions of hydrophobic substrates,particularly for enantioselective biosynthesis.

关 键 词：HP-β-CD Recombinant nitrilase 2-Amino phenylpropionitrile ENANTIOSELECTIVITY e.e.value 

分 类 号：O62[理学—有机化学]