hsa_circ_0023984在原发性肝癌中的表达及ceRNA网络的构建  

Expression of hsa_circ_0023984 in hepatic celluler cancer and construction of the ceRNA network

作　　者：覃如素 吴玲红 梁善雄 杨莉[1] 黄天壬[2] QIN Rusu;WU Linghong;LIANG Shanxiong;YANG Li;HUANG Tianren(Department of Public Health,Guangxi Medical University,Nanning 530021,Guangxi,China;Department of Experimental Research,Affiliated Tumor Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China;Department of Statistics,The Fourth Affiliated Hospital of Guangxi Medical University/Liuzhou Workers’Hospital,Liuzhou 545000,Guangxi,China;Medical Record Department,Affiliated Tumor Hospital of Guangxi Medical University,Nanning 530021,Guangxi,China)

机构地区：[1]广西医科大学公共卫生学院,广西南宁530021 [2]广西医科大学附属肿瘤医院实验研究部,广西南宁530021 [3]广西医科大学第四附属医院/柳州市工人医院统计科,广西柳州545000 [4]广西医科大学附属肿瘤医院病案科,广西南宁530021

出　　处：《中国现代医生》2025年第8期5-8,20,共5页China Modern Doctor

基　　金：2019年广西壮族自治区自然科学基金课题项目(2019JJD140017);广西壮族自治区卫生健康委员会自筹项目(Z20210032)。

摘　　要：目的探讨hsa_circ_0023984在原发性肝癌(hepatic celluler cancer,HCC)及癌旁组织的表达与临床意义,构建内源竞争性RNA调控网络。方法下载并处理基因扩展综合数据库中GES97332数据集肝癌组织和癌旁组织样本的circRNA表达谱,筛选差异表达circRNA。检测34例肝癌患者组织中hsa_circ_0023984的表达情况,分析其与临床病理特征间的关系。构建蛋白质相互作用网络,通过基因本体论(gene ontology,GO)和京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)富集分析进一步了解其功能。结果hsa_circ_0023984在肿瘤组织中较癌旁组织中低表达。hsa_circ_0023984表达水平与包膜情况有关(F=3.47,P<0.05)。共获得3个靶向miRNAs,Hub基因为PARGC1、GNG4、WNT5A、CDK6、SP1、KRAS、GNAI3、NCOA3、PIK3R1、MAPK8。GO分析与KEGG分析表明hsa_circ_0023984的下游mRNAs可能参与癌症通路与癌症的胆碱代谢通路。结论hsa_circ_0023984在HCC中高表达,hsa_circ_0023984的高表达可能与HCC恶性发展有关。Objective To explore the expression of hsa_circ_0023984 in hepatic celluler cancer(HCC)and its adjacent tissues and to analyze its clinical significance,and to construct an endogenous competitive RNA regulatory network.Methods CircRNA expression profiles of cancer tissue and adjacent tissue samples from GES97332 dataset in Gene Expession Omnibus database were downloaded and circRNA differential expression was screened.The expression of hsa_circ_0023984 was detected in the tissues of 34 HCC patients,and the relationship between it and the clinicopathological features was analyzed.The protein interaction network was constructed,and the function was further understood by gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis.Results The expression of hsa_circ_0023984 was lower in tumor tissues than in adjacent tissues.The expression level of hsa_circ_0023984 was related to the envelope condition(F=3.47,P<0.05).Three targeted miRNAs were obtained,and the Hub genes were PARGC1,GNG 4,WNT 5 A,CDK 6,SP 1,KRAS,GNAI 3,NCOA3,PIK3R1,and MAPK 8.GO analysis and KEGG analysis indicated that downstream mRNAs of hsa_circ_0023984 may be involved in cancer pathways and the choline metabolism pathway in cancer.Conclusion High expression of hsa_circ_0023984 in primary HCC,and high expression of hsa_circ_0023984 may be related to the malignant development of primary HCC.

关 键 词：原发性肝癌 circRNA hsa_circ_0023984 

分 类 号：R73[医药卫生—肿瘤]