表面活性剂协同红外辅助提取柴胡中柴胡皂苷a工艺优化及抗氧化性能研究  

Optimization of extraction process of saposide a from bupleurum with the aid of surfactants and infrared and study on antioxidant properties

作　　者：何敏 刘珂 张虹 郁静蕾 汪永祥 赵鑫 张欣怡 毕映燕[2] 易九州 HE Min;LIU Ke;ZHANG Hong;YU Jing-lei;WANG Yong-xiang;ZHAO Xin;ZHANG Xin-yi;BI Ying-yan;YI Jiu-zhou(Lanzhou Petrochemical University of Vocational Technology,Lanzhou 730060,China;Department of Pharmacy,Gansu Provincial Hospital of TCM,Lanzhou 730050,China;School of Chemistry and Chemical Engineering of Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州石化职业技术大学,甘肃兰州730060 [2]甘肃省中医院药剂科,甘肃兰州730050 [3]兰州大学化学化工学院,甘肃兰州730000

出　　处：《化学研究与应用》2025年第3期677-684,共8页Chemical Research and Application

基　　金：甘肃省高等学校创新基金项目(2023A-197)资助;兰州石化职业技术大学科研创新团队-绿色化工新材料科研创新团队(兰石化大学校发[2023]201号)资助。

摘　　要：利用表面活性剂辅助红外辐射提取方法提取柴胡中的柴胡皂苷a,采用单因素试验结合Box-Behnken响应面法优化提取工艺,通过测定提取物对ABTS^(+)及DPPH自由基的清除能力评价其体外抗氧化活性。单因素试验结果表明:AEO-9作为溶剂体积分数7.5%,液固比为40:1,红外功率250 W,提取时间60 min为最优提取条件;Box-Behnken响应面试验优化结果:AEO-9浓度、液固比、提取时间、红外功率4个提取条件优化结果分别为7.89%、33.28:1、70.13 min、229.35 W,该条件下SSa提取率为8.4221 mg·g^(-1),与理论预测值(8.4288 mg·g^(-1))相对误差仅为0.08%。体外抗氧化试验表明,该法获取的柴胡提取物对ABTS^(+)及DPPH自由基半数清除浓度(IC_(50))分别为1.02%及1.93%,表明其具有良好的抗氧化能力。Extraction of saikosaponin a from bupleurum by surfactants-assisted infrared radiation extraction method.The single factor combined with Box-Behnken response surface method was used to optimize the extraction process,and the antioxidant activity of the extracts was evaluated by measuring the scavenging ability of ABTS^(+)and DPPH free radicals.The results of single factor test showed that the optimal extraction conditions were as follows:the volume fraction of AEO-9 was 7.5%,the liquid-solid ratio was 40:1,the infrared power was 250 W,and the extraction time was 60 min.Response surface test results:The optimal results of the four factors were 7.89%,33.28:1,70.13 min and 229.35 W,respectively.Under these conditions,the extraction rate of SSa was 8.4221mg·g^(-1),and the theoretical predicted value was 8.4288 mg·g^(-1),the relative error was only 0.08%.The in vitro antioxidant test showed that the half scavenging concentration(IC_(50))of ABTS^(+)and DPPH free radicals of Bupleurum extract obtained by this method were 1.02%and 1.93%,respectively.

关 键 词：红外辐射 表面活性剂 柴胡皂苷A 响应面法 抗氧化 

分 类 号：O29.9[理学—应用数学]