基于硫、氧掺杂荧光碳点的尿酸测定方法研究  

Determination method of uric acid based on sulfur and oxygen doped fluorescent carbon dots

作　　者：信明缘 经梦珂 张翠利[1] 信建豪[1] XIN Ming-yuan;JING Meng-ke;ZHANG Cui-li;XIN Jian-hao(School of Medicine,Yellow River University of Science and Technology,Zhengzhou 450063,China)

机构地区：[1]黄河科技学院医学部,河南郑州450063

出　　处：《化学研究与应用》2025年第3期743-747,共5页Chemical Research and Application

基　　金：河南省研究生联合培养基地项目(YJS2024JD57)资助;河南省大学生创新创业项目(202311834031)资助;郑州市医疗卫生领域科技创新指导计划(2024YLZDJH021)资助;郑州市基础研究与应用基础研究项目(ZZSZX202424)资助;郑州市基础研究与应用基础研究项目(ZZSZX202420)资助。

摘　　要：建立一种基于硫、氧掺杂碳量子点的尿酸荧光测定方法。以乙二胺四乙酸二钠和硫脲为原料固相反应制备硫、氧掺杂的荧光碳量子点(S,O-CNQDs),然后在制备的碳量子点溶液中加入KI,Cu(NO_(3))_(2)溶液使碳点荧光猝灭,最后加入尿酸可使荧光再次恢复,加入尿酸的量与恢复的荧光值存在线性关系。结果表明,在pH为4.0,KI(4.00mmol·L^(-1))、Cu(NO_(3))_(2)(1.00mmol·L^(-1))溶液为4.00mL,反应时间为15分钟时荧光值与尿酸的浓度(0.01mmol·L^(-1)-1.00mmol·L^(-1))之间存在良好的线性关系,线性回归方程为F=234.7+327c,R=0.998。用于测定尿酸的含量,回收率在93.1%~105.6%。方法快速准确,可用于实际样品的测定。A fluorescent determination method for uric acid based on sulfur and oxygen-doped carbon quantum dots was established.Sulfur and oxygen-doped fluorescent carbon quantum dots(S,O-CNQDs)were synthesized through a solid-phase reaction using ethylene diamine tetraacetic acid disodium salt and thiourea as raw materials.Subsequently,KI and Cu(NO_(3))_(2)solutions were added to the prepared carbon quantum dot solution to quench the fluorescence of the carbon dots.Finally,the addition of uric acid restored the fluorescence,and the amount of uric acid added was linearly related to the recovered fluorescence value.The results indicated that at pH 4.0,with KI(4 mmol·L^(-1))and Cu(NO_(3))_(2)(1 mmol·L^(-1))solutions at 4 mL,and a reaction time of 15 minutes,there was a good linear relationship between the fluorescence value and the concentration of uric acid(0.01 mmol·L^(-1)to 1 mmol·L^(-1)).The linear regression equation was F=234.7+327c,with a correlation coefficient R of 0.998.The method was used to determine the content of uric acid,with a recovery rate ranging from 93.1%to 105.6%.This method was rapid and accurate,suitable for the determination of uric acid in actual samples.

关 键 词：碳点 尿酸 测定 掺杂 

分 类 号：O652.1[理学—分析化学]