细脚虫草多糖发酵条件优化及抗氧化活性  

Optimization of fermentation conditions and antioxidant activity of polysaccharides from Cordyceps tenuipes

作　　者：李玉婷 陈梦 李安妮 陈志豪 夏艳秋 朱强 LI Yuting;CHEN Meng;LI Anni;CHEN Zhihao;XIA Yanqiu;ZHU Qiang(School of Marine Food and Biological Engineering,Jiangsu Ocean University,Lianyungang 222005,China;Lianyungang Food and Drug Inspection and Testing Center,Lianyungang,222000,China)

机构地区：[1]江苏海洋大学海洋食品与生物工程学院,江苏连云港222005 [2]连云港市食品药品检验检测中心,江苏连云港222000

出　　处：《江苏海洋大学学报(自然科学版)》2025年第1期31-40,共10页Journal of Jiangsu Ocean University:Natural Science Edition

摘　　要：采用水提醇沉工艺提取细脚虫草多糖(Cordyceps tenuipes polysaccharides,CTP),以多糖含量为响应值,在单因素试验基础上,利用Box-Behnken中心组合进行四因素三水平试验设计优化细脚虫草多糖发酵培养工艺,并考察分级醇沉后多糖的体外抗氧化活性。最佳培养基组成为:蔗糖4%,蛋白胨1.5%,磷酸二氢钾0.12%和水蛭0.46%;同时接种量7.3%、装液量36%,初始pH4.2,发酵温度25℃和发酵时间9d,在此条件下菌丝体粗多糖含量为62.08 mg/g,比优化前提高了16.58%。多糖体外抗氧化试验显示,分级醇沉后的CTP70组分的抗氧化效果最佳,且相较于优化前的CTP0组分,在5 mg/mL的浓度下对DPPH自由基清除率提高了22.54%、·OH自由基清除率提高了22.38%,铁离子还原能力提高了51.78%。实验表明,添加水蛭优化后的细脚虫草发酵培养条件有效提高细脚虫草菌丝体多糖产量,同时,CTP70组分表现出良好的抗氧化活性。ion and alcohol precipitation,the polysaccharide content was used as the response value,and the fermentation process of Cordyceps tenuipes polysaccharides was optimized by Box-Behnken central combination on the basis of single factor test,and the antioxidant activity of polysaccharides after graded alcohol precipitation was investigated.The optimal medium composition was:4%of sucrose,1.5%of protein,0.12%of KH_(2)PO_(4)and 0.46%of leech.While the inoculum was 7.3%,liquid filling volume was 36%,initial pH was 4.2,25℃and time of 9 d,the mycelia polysaccharides content was 62.08 mg/g,which was 16.58%higher than before.The in vitro antioxidant test of polysaccharides showed that the CTP70 component after graded alcohol precipitation had the best antioxidant effect.Compared with the CTPO component before optimization,at a concentration of 5 mg/mL,the DPPH radical scavenging rate increased by 22.54%,the·OH radical scavenging rate increased by 22.38%,and the iron ion reduction ability increased by 51.78%.The experiments showed that the optimized fermentation culture conditions with leech addition effectively improved the mycelia polysaccharides production,and CTP70 exhibited good antioxidant activity.

关 键 词：细脚虫草 多糖 抗氧化活性 响应面 

分 类 号：Q815[生物学—生物工程]