荧光光谱法测定食品中痕量甲醛  

Catalytic Kinetic Fluorescence Spectrometry Determination of Formaldehyde in Food

作　　者：陈浩 宋丹丹[1] 曾艳红 刘文虎 CHEN Hao;SONG Dandan;ZENG Yanhong;LIU Wenhu(Yueyang Vocational Technical College,Yueyang,Hunan 414000;Xiangtan University,Xiangtan,Hunan 411105;Hunan Petrochemical Vocational Technology College,Yueyang,Hunan 414000)

机构地区：[1]岳阳职业技术学院,湖南岳阳414000 [2]湘潭大学,湖南湘潭411105 [3]湖南石油化工职业技术学院,湖南岳阳414000

出　　处：《岳阳职业技术学院学报》2025年第1期73-77,共5页Journal of Yueyang Vocational and Technical College

基　　金：2022年度湖南省教育厅科学研究一般项目“水质中微量游离氯及甲醛检测的功能化试纸研究”(22C1367);2023年度湖南省教育厅科学研究优秀青年项目“生物质材料浸出锂离子电池正极材料有价金属回收的研究”(23B1099)。

摘　　要：基于磷酸介质中甲醛对溴酸钾氧化伊红Y褪色的催化作用,通过测定伊红Y的荧光光谱的变化,建立催化动力学荧光光谱法测定痕量甲醛的新方法,相比分光光度法具有更高灵敏度。最佳条件为:伊红Y浓度1.16×10^(-6)mol/L,溴酸钾浓度0.007mol/L,pH=1.7,在90℃水浴中反应10min,甲醛在0.005~0.06μg/mL范围内与伊红Y荧光强度差值△F呈良好线性关系,线性方程为△F=27.55+9643.48C_(FA)(R^(2)=0.9991,n=12),相关系数R^(2)=0.9991,甲醛浓度的最低检出限为3.13×10^(-5)μg/mL,将检测方法应用于黑木耳和腐竹中甲醛浓度的测定,取得了满意的检测结果。Based on the catalytic action of trace formaldehyde on the oxidation of eosin Y by potassium bromate in phosphoric acid medium,a new catalytic kinetic fluorescence method for the determination of trace formaldehyde was developed.Compared with the spectrophotometric method,it has higher sensitivity.Under the optimal experimental-6 conditions:the concentration of eosin Y is 1.16×10^(-6) mol/L,the concentration of potassium bromate is 0.007 mol/L,pH=1.7,and the reaction is carried out at 90 C water bath for 10 minutes.Formaldehyde shows good linear relationship with the fluorescence intensity difference△F in the range of 0.005-0.06μg/mL,the linear equation was △F=27.55+9643.48CFA(R^(2)=0.9991,n=12),and the correlation coefficient was 0.9991 with the limit of detection of 3.13×10^(-5)μg/mL.The experimental method was applied to the determination of formaldehyde content in black fungus and yuba,and satisfactory results were obtained.

关 键 词：伊红Y 溴酸钾 荧光法 甲醛 

分 类 号：TS207.5[轻工技术与工程—食品科学]