两型花植物孩儿参不同发育期内源激素水平的变化  

Variation of endogenous hormone levels of different development stages in dimorphic flower plant Pseudostellaria heterophylla

作　　者：苏诗越 胡文昭 张硕 张丽辉 张彦文 SU Shiyue;HU Wenzhao;ZHANG Shuo;ZHANG Lihui;ZHANG Yanwen(College of Biological Sciences,Changchun Normal University,Changchun 130032,Jilin,China;School of Agricultural,Liaodong University,Dandong 118003,Liaoning,China)

机构地区：[1]长春师范大学生命科学学院,吉林长春130032 [2]辽东学院农学院,辽宁丹东118003

出　　处：《生物资源》2025年第1期40-48,共9页Biotic Resources

基　　金：国家自然科学基金项目(31370400)。

摘　　要：具有顺序性繁殖输出特征的两型花植物内源激素水平与发育期的关系目前了解较少。以典型两型花植物孩儿参为材料,采用高效液相色谱法检测了该植物6个发育阶段块根中4种内源激素水平并计算了相互间的比值。结果显示,赤霉素(GA_(3))含量在不同发育阶段呈上升趋势,并在闭锁花果实成熟期达到高峰,但在块根成熟期又恢复到较低水平;脱落酸(ABA)呈现两个峰值,峰值1出现在开放花盛期,峰值2在闭锁花盛期;吲哚乙酸(IAA)在发育的中前期呈现稍微降低的趋势,但在闭锁花种子成熟期达到高峰;玉米素(ZT)在发育的中前期较稳定,但在闭锁花盛期达到高峰,随后开始下降。4组内源激素的比值(GA_(3)/IAA,ABA/ZT,ABA/IAA,IAA/ZT)显示,其与开放花和闭锁花的开花频率、开花时间以及块根的形成有紧密关系,影响了该种的顺序性的繁殖输出模式。There is limited understanding of the relationship between endogenous hormone levels and development stages in dimorphic flower plants with sequential reproduction output characteristics.In this study,Pseudostellaria heterophylla,a typical dimorphic flower plant,was selected as the material,and the levels of 4 endogenous hormones in root tubers at 6 developmental stages were measured by high performance liquid chromatography(HPLC)and their ratios were calculated.The results showed that gibberellin A_(3)(GA_(3))content increases at different developmental stages,and reaches its peak at the mature stage of cleistogamous flower fruit,but returns to a lower level at the maturing stage of root tubers.There are two peak values of abscisic acid(ABA),the peak value 1 appears at the peak stage of chasmogamous flower,and the peak value 2 at the peak stage of cleistogamous flower.Auxin(IAA)decreases slightly in the middle and early stages of development,but reaches its peak in the mature stage of cleistogamous flower seeds.Zeatin(ZT)is stable in the middle and early stages of development,but reaches its peak in the flowering stage of cleistogamous flowers and then begins to decline.The ratios of endogenous hormones(GA_(3)/IAA,ABA/ZT,ABA/IAA,IAA/ZT)of the four groups showed a close relationship with the flowering frequency,flowering time,and the formation of tuber roots of chasmogamous and cleistogamous flowers,affecting the sequential reproductive output pattern of this species.

关 键 词：孩儿参 内源激素 开放花 闭锁花 顺序性繁殖输出 

分 类 号：Q945.79[生物学—植物学]