Improvement of Lactiplantibacillus plantarum MWFLp-182 on oxidative deficits induced by in 2,2'-azobis(2-methylpropionamidine)dihydrochloride and the relating key gene analysis  

作　　者：Hui Nie Xutong Ma Fanyu Kong Yanghe Luo Guangqing Mu Xiaomeng Wu 

机构地区：[1]School of Food Science and Technology,Dalian Polytechnic University,Dalian 116034,China [2]Dalian Probiotic Function Research Key Laboratory,Dalian Polytechnic University,Dalian 116034,China [3]Guangxi Key Laboratory of Health Care Food Science and Technology,Hezhou University,Hezhou 542899,China

出　　处：《Food Science and Human Wellness》2025年第2期497-508,共12页食品科学与人类健康(英文)

基　　金：supported by the Natural Science Foundation of Guangxi,China(2020GXNSFBA297083);the National Natural Science Foundation of China(32072193)。

摘　　要：Lactiplantibacillus plantarum MWFLp-182 was originally isolated from the feces of long-living elderly individuals in Hezhou city,Guangxi,China.L.plantarum MWFLp-182 showed favorable gastric and intestinal tolerance compared with Lactiplantibacillus rhamnosus GG(LGG).L.plantarum MWFLp-182 was further examined for its antioxidant and anti-inflammatory activity in ABAP-treated HT-29 cells.Importantly,it enhanced the expression of anti-inflammatory factor(interleukin-10(IL-10)),antioxidant cytokines(superoxide dismutase(SOD),glutathione peroxidase(GPx),nuclear factor erythroid 2-related factor(Nrf2),and hemeoxygenase 1(HO-1)),B-cell lymphoma-2(Bcl-2),and tight junction(Zonula occludens protein 1(ZO-1),Claudin-1,and Occludin)proteins and genes,and reduced reactive oxygen species(ROS).The complete genome of L.plantarum MWFLp-182 contained a single circular chromosome that was 3257196 bp long,with a G+C content of 44.49%,and a single circular plasmid that was 53560 bp long.The genes tpx,trx A,trx B,npr,nrd H,dps,rec A,gpx,gsh A and ars C in this strain were related to antioxidant function;the key genes involved in antioxidant function were further investigated.In this study,we identified a probiotic candidate with antioxidant properties.

关 键 词：Lactiplantibacillus plantarum Antioxidant strain Complete genome sequence 

分 类 号：R73[医药卫生—肿瘤]