Inulin alleviates HFD-induced NAFLD by hepatic macrophage polarization and lipid metabolism via gut-liver axis  

作　　者：Wei Song Jinhong Hu Lina Zhu Bing Chen Lingling Li Xiaole Cui Ting Zhang Yaxing Miao Kaixin Su Changchun Liu Yane Luo Tianli Yue 

机构地区：[1]College of Food Science and Technology,Laboratory of Nutritional and Healthy Food-Individuation Manufacturing Engineering,Research Center of Food Safety Risk Assessment and Control,Northwest University,Xi’an 710069,China

出　　处：《Food Science and Human Wellness》2025年第2期633-650,共18页食品科学与人类健康(英文)

基　　金：supported by the projects of the National Natural Science Foundation of China(31901701);the National Key Research and Development Program of China(2019YFC1606704);Key R&D Program Project of Shaanxi Province(2024NC-YBXM-151-4);Science and Technology Plan Project of Xi'an(2014JH-NYYB-0103)。

摘　　要：Long-term intake of high-fat diet(HFD)leads to systemic hypo-inflammation,which is involved in the pathogenesis of many liver diseases.The aim of this study was to evaluate the interventional effect of inulin addition on HFD-induced non-alcoholic fatty liver disease(NAFLD).Here,we assessed liver inflammation and lipid metabolism by enzyme linked immunosorbent assay(ELISA)kits and real-time quantitative polymerase chain reaction(q PCR),and explored its relationship with intestinal flora and metabolites by 16S r DNA gene sequencing and untargeted metabolomics.In addition,we determined the effect of the inositol-requiring enzyme-1α-X-box binding protein 1(IRE1α-XBP1)pathway on liver macrophage polarization by flow cytometry.In conclusion,our study has demonstrated that inulin ameliorates high levels of bile acids and colonic immune disorders induced by HFD,and improves hepatic macrophage polarization and hepatic lipid metabolism via the intestine-hepatic axis,thus providing a mechanistic basis for therapeutic approaches to modulate bile acids to improve colonic inflammation and NAFLD.

关 键 词：High-fat diet non-alcoholic fatty liver disease Macrophage polarization Bile acid IRE1α-XBP1 pathway 

分 类 号：R57[医药卫生—消化系统]