Molecular basis of the allergenicity and cross-reactivity of a major allergen-Crassostrea gigas arginine kinase  

作　　者：Yanbo Wang Peiao Zhang Jinru Zhou Jihui Gao Jinlyu Sun Di Wu Wentong Xue Dong Yang Linglin Fu 

机构地区：[1]Food Safety Key Laboratory of Zhejiang Province,School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China [2]College of Food Science&Nutritional Engineering,China Agricultural University,Beijing 100083,China [3]Allergy Department,State Key Laboratory of Complex Severe and Rare Diseases,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730,China [4]Institute for Global Food Security,School of Biological Sciences,Queen’s University of Belfast,Belfast BT95DL,United Kingdom

出　　处：《Food Science and Human Wellness》2025年第4期1281-1290,共10页食品科学与人类健康(英文)

基　　金：financially supported by the State key research and development plan(2019YFC1605000);the National Natural Science Foundation of China(31871735)。

摘　　要：As an important shellfish group,the oyster can induce severe allergic reactions.We aimed to identify and characterize the major allergen in Crassostrea gigas,and elucidate the molecular basis of its allergenicity and cross-reactivity.The native and recombinant C.gigas-arginine kinase(AK)displayed significant immunoglobulin(Ig)G-and Ig E-binding activity.The Ig E-binding activity of C.gigas-AK could be reduced by thermal treatment and strong acidic and alkaline conditions.Besides,cross-reactivity of AK was demonstrated between shellfish species.Among seven epitope peptides identified here,P2 is responsible for the specificity of C.gigas-AK,while P3 is responsible for cross-reactions between mollusks and crustaceans.Furthermore,Glu98 and His31 in the light chain,Arg101,and Lys57 in the heavy chain are identified as key Ig E residues in recognizing epitopes.These findings provide new insights into the prevention and treatment of shellfish allergy and the development of hypoallergenic shellfish products.

关 键 词：Oyster allergy Crassostrea gigas CROSS-REACTIVITY Arginine kinase 

分 类 号：Q93[生物学—微生物学]