Screening of Chinese giant salamander meat hydrolysates with DPP-Ⅳinhibitory activity and systematic elucidation of their hypoglycemic functions in mouse model  

作　　者：Shucheng Li Changge Guan Yi Wang Haihong Chen Wei Li Songjun Wang Chong Zhang Xinhui Xing 

机构地区：[1]Key Laboratory for Industrial Biocatalysis,Ministry of Education of China,Department of Chemical Engineering,Tsinghua University,Beijing 100084,China [2]School of Life Science and Technology,Tokyo Institute of Technology,Yokohama 225-8501,Japan [3]Institute of Biopharmaceutical and Health Engineering,Tsinghua Shenzhen International Graduate School,Shenzhen 518055,China [4]Henan Giant Salamander Protection and Development Association,Luoyang 471000,China. [5]Center for Synthetic and Systems Biology,Tsinghua University,Beijing100084,China [6]Institute of Biomedical Health Technology and Engineering,Shenzhen Bay Laboratory,Shenzhen 518055,China

出　　处：《Food Science and Human Wellness》2025年第4期1305-1317,共13页食品科学与人类健康(英文)

基　　金：funded by the Shenzhen Agricultural Development Special Fund(Fishery)Agricultural High-tech Project([2021]735);SAMR technical guarantee project(2023YJ22 and 2023YJ37)。

摘　　要：Type 2 diabetes(T2D)presents a significant health challenge,underscoring the need for functional foods and nutraceutical hypoglycemic bioactive peptides for its prevention.This study investigates the potential of proteolytic hydrolysate from artificially cultivated Chinese giant salamander(CGS)meat,a rich protein source,as a preventive strategy for T2D.We produced a CGS meat hydrolysate(CGSh)and demonstrated its ability to inhibit the T2D drug target dipeptidyl peptidaseⅣ(DPP-Ⅳ)through in vitro assays.We identified 5 peptides(WRPPDH,WAPPSKD,IPDSPF,IPEMIF,and VPIAVPT)with high DPP-Ⅳinhibitory activity in CGSh,suggesting its potential antidiabetic effects.In vivo experiments showed that CGSh effectively reduced insulin resistance in mice induced with a high-fat diet,as evidenced by a slower increase in blood glucose levels and a decreased HOMA-IR index.16S rRNA sequencing analysis revealed that CGSh improved gut microbial homeostasis,promoting beneficial microorganisms and reducing harmful bacteria.Metabolomic analyses identified an increase in valeric acid levels and highlighted nine potential biomarker metabolites.By inhibiting metabolic pathways such as AGE-RAGE,CGSh might also prevent diabetic complications and reduce inflammation.These findings suggest that CGSh has a promising hypoglycemic effect,making it a potential functional food ingredient for T2D prevention and treatment.

关 键 词：Chinese giant salamander Hypoglycemic effects Bioactive peptides Dipeptidyl dipeptidaseⅣ Multi-omics analysis 

分 类 号：R58[医药卫生—内分泌]