Immobilization of Thermomyces lanuginosus lipase in a novel polysaccharide-based hydrogel by a two-step crosslinking method and its use in the lauroylation ofα-arbutin  

作　　者：Ming Chen Weina She Xin Zhao Cheng Chen Benwei Zhu Yun Sun Zhong Yao 

机构地区：[1]College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211816,China [2]Department of Chemical and Pharmaceutical Engineering,Southeast University Chenxian College,Jiangsu,China.

出　　处：《Bioresources and Bioprocessing》2024年第1期83-96,共14页生物资源与生物加工(英文)

基　　金：supported by the Youth Foundation of Southeast University ChengXian College(z0055).

摘　　要：The Thermomyces lanuginosus lipase(TLLs)was successfully immobilized within a novel hydrogel matrix through a two-step crosslinking method.TLLs were initially crosslinked through the Schiff base reaction by oxidized carboxymethyl cellulose(OCMC).The water-soluble OCMC@TLLs complex was subsequently crosslinked by carboxymethyl chitosan(CMCSH)in a microfluidic apparatus to form the CMCHS/OCMC@TLLs microspheres.The CD(Circular Dichroism,CD)and FT-IR(Fourier Transform infrared spectroscopy,FT-IR)spectra demonstrated that the crosslinking of TLLs with OCMC resulted in a less significant impact on their structure compared to that with glutaraldehyde.CMCHS/OCMC@TLLs showed decreased catalytic performance due to the mass transfer resistance,while its thermal stability was greatly improved.The CMCHS/OCMC@TLLs were used to catalyze the lauroylation of arbutin in tetrahydrofuran.After 12 h of reaction under optimal conditions,the yield of 6′-O-lauryl arbutin reached an impres-sive 92.12%.The prepared 6′-O-lauryl arbutin has high lipophilicity and exhibits similar tyrosinase inhibitory activity and higher antioxidant activity compared to its parent compound.

关 键 词：ARBUTIN Oxidized carboxymethyl cellulose Carboxymethyl chitosan Microfluidic Covalent crosslinked polymer LIPASE ACYLATION 

分 类 号：O63[理学—高分子化学]