Production of biliverdin by biotransformation of exogenous heme using recombinant Pichia pastoris cells  

作　　者：Jianfeng Mei Yanchao Han Shihang Zhuang Zhikai Yang Yu Yi Guoqing Ying 

机构地区：[1]College of Pharmaceutical Science,Zhejiang University of Technology,18 Chaowang Road,Gongshu District,Hangzhou 310014,Zhejiang,China.

出　　处：《Bioresources and Bioprocessing》2024年第1期263-272,共10页生物资源与生物加工(英文)

基　　金：financially supported by the Key Research and Development Program of Zhejiang Province(2021C03088-2).

摘　　要：Biliverdin,a bile pigment hydrolyzed from heme by heme oxygenase(HO),serves multiple functions in the human body,including antioxidant,anti-inflammatory,and immune response inhibitory activities.Biliverdin has great potential as a clinical drug;however,no economic and efficient production method is available currently.Therefore,the production of biliverdin by the biotransformation of exogenous heme using recombinant HO-expressing yeast cells was studied in this research.First,the heme oxygenase-1 gene(HO1)encoding the inducible plastidic isozyme from Arabidopsis thaliana,with the plastid transport peptide sequence removed,was recombined into Pichia pasto-ris GS115 cells.This resulted in the construction of a recombinant P.pastoris GS115-HO1 strain that expressed active HO1 in the cytoplasm.After that,the concentration of the inducer methanol,the induction culture time,the pH of the medium,and the concentration of sorbitol supplied in the medium were optimized,resulting in a significant improvement in the yield of HO1.Subsequently,the whole cells of GS115-HO1 were employed as catalysts to convert heme chloride(hemin)into biliverdin.The results showed that the yield of biliverdin was 132 mg/L when hemin was added to the culture of GS115-HO1 and incubated for 4 h at 30°C.The findings of this study have laid a good foundation for future applications of this method for the economical production of biliverdin.

关 键 词：BILIVERDIN BIOTRANSFORMATION HEME Heme oxygenase-1 Recombinant Pichia pastoris 

分 类 号：F42[经济管理—产业经济]