Enhancement of astaxanthin accumulation via energy reassignment by removing the flagella of Haematococcus pluvialis  

作　　者：Yuyong Hou Zhile Guo Zhiyong Liu Suihao Yan Meijie Cui Fangjian Chen Weijie Wang Longjiang Yu Lei Zhao 

机构地区：[1]College of Life Science,North China University of Science and Technology,Tangshan,China [2]Photosynthesis Research Center,Key Laboratory of Photobiology,Institute of Botany,Chinese Academy of Sciences,Beijing,China [3]Key Laboratory of Engineering Biology for Low-carbon Manufacturing,Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin,China [4]National Center of Technology Innovation for Synthetic Biology,Tianjin,China [5]University of Chinese Academy of Sciences,Beijing,China

出　　处：《Bioresources and Bioprocessing》2024年第1期1041-1046,共6页生物资源与生物加工(英文)

基　　金：supported by the National Key R&D Program of China(2022YFC3401803);the Strategic Priority Research Program of Chinese Academy of Sciences(XDC0120301);the Hundred Talents Program of the Chinese Academy of Sciences(E3J56201);the Science and Technology Partnership Program,Ministry of Science and Technology of China(KY202001017);the Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project(TSBICIP-IJCP-001,TSBICIP-IJCP-002,TSBICIP-CXRC-027).

摘　　要：Astaxanthin biosynthesis in Haematococcus pluvialis is driven by energy.However,the effect of the flagella-mediated energy-consuming movement process on astaxanthin accumulation has not been well studied.In this study,the profiles of astaxanthin and NADPH contents in combination with the photosynthetic parameters with or without flagella enabled by pH shock were characterized.The results demonstrated that there was no significant alteration in cell morphology,with the exception of the loss of flagella observed in the pH shock treatment group.In contrast,the astaxanthin content in the flagella removal groups was 62.9%,62.8%and 91.1%higher than that of the control at 4,8 and 12 h,respectively.Simultaneously,the increased Y(II)and decreased Y(NO)suggest that cells lacking the flagellar movement process may allocate more energy towards astaxanthin biosynthesis.This finding was verified by NADPH analysis,which revealed higher levels in flagella removal cells.These results provide preliminary insights into the underlying mechanism of astaxanthin accumulation enabled by energy reassignment in movement-lacking cells.

关 键 词：Haematococcus pluvialis ASTAXANTHIN FLAGELLA Energy flow pH-shock 

分 类 号：Q94[生物学—植物学]