ZnFe_(2)O_(4)@Ag纳米复合材料的制备及其抑菌性能  

Preparation and antibacterial properties of ZnFe_(2)O_(4)@Ag nanocomposites

作　　者：张艳艳[1] 房宇[1] 张萌萌 张滢 吴鹏[1] 魏艳妮 刘晓琴[1] 刘嘉华 丁佳睿 谭玉婷 郭少波 ZHANG Yanyan;FANG Yu;ZHANG Mengmeng;ZHANG Ying;WU Peng;WEI Yanni;LIU Xiaoqin;LIU Jiahua;DING Jiarui;TAN Yuting;GUO Shaobo(School of Pharmacy,Hanzhong Vocational and Technical College,Hanzhong 723001,China;College of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong 723000,China;School of Chemistry and Environmental Science,Shaanxi University of Technology,Hanzhong 723000,China)

机构地区：[1]汉中职业技术学院药学院,汉中723001 [2]陕西理工大学生物科学与工程学院,汉中723000 [3]陕西理工大学化学与环境科学学院,汉中723000

出　　处：《复合材料学报》2025年第2期1092-1104,共13页Acta Materiae Compositae Sinica

基　　金：陕西省教育厅专项科研计划项目(19JK0050)。

摘　　要：由于传统抗生素和抗菌剂的过度使用,导致大量耐药菌滋生,对社会公共安全和人类健康产生严重威胁。因此,迫切需要开发新一代的抗菌材料来应对耐药菌危害。本文以三氯化铁(FeCl+(3))、氯化锌(ZnCl_(2))和醋酸钠(NaOAc)为原料,采用“一锅法”合成磁性ZnFe_(2)O_(4)纳米微球,并把平均粒径尺寸为8.8 nm的银纳米颗粒(Ag NPs)吸附到ZnFe_(2)O_(4)表面,制备得到ZnFe_(2)O_(4)@Ag磁性纳米复合材料。该材料可有效防止Ag NPs的团聚,同时小粒径的纳米银可大幅度提升复合材料的抑菌活性,且Zn和Fe元素的引入可提升生物相容性。采用TEM、XPS、XRD、UV-Vis、FTIR及VSM等对复合材料进行系统表征。以革兰氏阴性菌大肠杆菌(E.coli)、革兰氏阳性金黄色葡萄球菌(S.aureus)为测试菌,研究复合材料的抑菌活性和抑菌机制。实验结果表明:ZnFe_(2)O_(4)@Ag在浓度为200μg/mL时,60 min内对E.coli和S.aureus的抑菌活性可达到99.9%,抑菌机制显示:ZnFe_(2)O_(4)@Ag破坏细菌细胞壁与细胞膜,使细菌内溶物及离子泄露,从而使细菌渗透压失衡,导致细菌死亡。同时该复合材料的生物相容性较Ag NPs也大幅度提升。The overuse of traditional antibiotics and antimicrobial agents has led to the growth of a large number of drug-resistant bacteria,which represents a serious threat to public safety and human health.Therefore,there is an urgent need to develop new-generation antimicrobial materials to cope with the hazards posed by drug-resistant bacteria.In this study,magnetic ZnFe_(2)O_(4)nanorods were synthesised using ferric chloride(FeCl_(3)),zinc chloride(ZnCl_(2))and sodium acetate(NaOAc)as raw materials.Silver nanoparticles(Ag NPs)with an average particle size of 8.8 nm were then adsorbed onto the surface of ZnFe_(2)O_(4),resulting in the formation of ZnFe_(2)O_(4)@Ag magnetic nanocomposites.This material can effectively prevent the agglomeration of Ag NPs,while the small diameter of the nanoparticles significantly enhances the antibacterial activity of the composite material,and the introduction of Zn and Fe elements can improve biocompatibility.The composite material was systematically characterized using TEM,XPS,XRD,UV-Vis,FTIR,and VSM,among others.The antibacterial activity and mechanism of the composite material were studied against Gram-negative Escherichia coli(E.coli)and Gram-positive Staphylococcus aureus(S.aureus)as test bacteria.Experimental results show that ZnFe_(2)O_(4)@Ag has an antibacterial activity of 99.9% against E.coli and S.aureus within 60 min at a concentration of 200μg/mL.The mechanism of antibacterial action indicates that ZnFe_(2)O_(4)@Ag disrupts the bacterial cell walls and membranes,causing leakage of bacterial contents and ions,thereby leading to osmotic imbalance and resulting in bacterial death.At the same time,the biocompatibility of this composite material is significantly improved compared to Ag NPs alone.

关 键 词：纳米材料 ZnFe_(2)O_(4)@Ag 抑菌 抑菌机制 磁分离 

分 类 号：O614[理学—无机化学] TB333[理学—化学]