汉黄芩苷对高脂饮食诱导的糖尿病大鼠肝损伤的保护作用  

Protective effect of Wogonoside on hepatic injury induced by high-fat diet in diabetic rats

作　　者：袁月 王丹鹤 李淑静[2] 仵玉玲 董欣 Yuan Yue;Wang Danhe;Li Shujing;Wu Yuling;Dong Xin(Department of Endocrinology,Beijing-Chaoyang Hospital,Capital Medical University,Beijing 100020,China;Disinfection Center,Beijing-Chaoyang Hospital,Capital Medical University,Beijing 100020,China;Department of Urology,Beijing-Chaoyang Hospital,Capital Medical University,Beijing 100020,China)

机构地区：[1]首都医科大学附属北京朝阳医院内分泌科,北京100020 [2]首都医科大学附属北京朝阳医院消毒中心,北京100020 [3]首都医科大学附属北京朝阳医院泌尿外科,北京100020

出　　处：《解剖学杂志》2025年第1期23-27,共5页Chinese Journal of Anatomy

基　　金：北京市自然科学基金青年项目(7184214)。

摘　　要：目的 :探讨汉黄芩苷(WO)对高脂饮食诱导的糖尿病(DM)大鼠肝损伤的保护作用及机制。方法 :将雄性Wistar大鼠随机分成对照组、模型(DM)组及3个不同浓度给药组(DM+WO 2、4、8 mg/kg)。通过腹腔注射30 mg/kg链脲佐菌素构建DM大鼠模型。ELISA检测总胆固醇(TC)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、超氧化物歧化酶(SOD)和丙二醛(MDA);免疫印迹测定肝组织凋亡相关蛋白及脂质代谢相关蛋白的表达;H-E染色观察肝组织病理学改变;TUNEL染色观察肝细胞凋亡情况。结果 :与对照组相比,模型组大鼠出现DM“三多一少”症状及肝病理损伤。与模型组相比,给药组的生化指标AST、ALT、TC、TG、LDL-C、MDA水平明显降低,而HDL-C、SOD水平明显升高;肝组织Bax/Bcl-2比值和乙酰辅酶A羧化酶的表达显著降低,c-Myc和肉毒碱棕榈酰转移酶1的表达显著升高;各给药组肝组织病理形态有所改善且肝细胞凋亡状态有所减轻,其中DM+WO 8 mg/kg组仅见少量凋亡细胞。结论 :WO对高脂饮食诱导的DM大鼠肝损伤具有一定的保护作用。Objective:To investigate the protective effect and mechanism of Wogonoside(WO)on hepatic injury induced by high-fat diet in diabetic rats.Methods:A total of 50 male Wistar rats were randomly divided into control group,model group(DM)and three drug administration groups(DM+WO 2,4 and 8 mg/kg).The diabetic rat model was established by intraperitoneal injection of 30 mg/kg streptozotocin.Total cholesterol(TC),alanine aminotransferase(ALT),aspartate aminotransferase(AST),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),superoxide dismutase(SOD),and malondialdehyde(MDA)were detected by ELISA.The expression levels of apoptosis-related proteins and lipid metabolism-related proteins in liver tissues were determined by Western blotting.H-E staining was used to observe the histopathological changes of liver.Apoptosis of hepatocytes was assessed by TUNEL staining.Results:Compared with the control group,the rats in the DM group showed typical symptoms of diabetes(frequent urination,thirst,hunger,and weight loss)and hepatic pathological injury.Compared with the DM group,the levels of biochemical indicators such as AST,ALT,TC,TG,LDL-C and MDA were obviously decreased in the drug administration groups,while the levels of HDL-C and SOD were obviously increased.The Bax/Bcl-2 ratio and acetyl-CoA carboxylase expression in liver tissue were significantly reduced,while the expression levels of c-Myc and carnitine palmitoyltransferase 1 were significantly elevated in the drug administration groups.The histopathological morphology of the liver was improved and the apoptosis of liver cells was reduced in the three WO treatment group,among which only a small number of apoptotic cells were observed in DM+WO 8 mg/kg group.Conclusion:WO has a certain protective effect on liver injury induced by high-fat diet in diabetic rats.

关 键 词：汉黄芩苷 糖尿病 肝损伤 谷草转氨酶 凋亡 

分 类 号：R362[医药卫生—病理学]