Temporal control in shell–core structured nanofilm for tracheal cartilage regeneration:synergistic optimization of anti-inflammation and chondrogenesis  

作　　者：Wen Zhao Fanglan Xu Yumei Shen Qifeng Ding Yifei Wang Leilei Liang Wufei Dai Yongbing Chen 

机构地区：[1]Department of Thoracic Surgery,The Second Affiliated Hospital of Soochow University,Suzhou,215004,China [2]Department of Thoracic Surgery,Tongren Hospital,Shanghai Jiao Tong University,School of Medicine,Shanghai,200050,China [3]Operation Room Department,The Second Affiliated Hospital of Soochow University,Suzhou,215004,China [4]Department of Gynecologic Oncology,Zhejiang Cancer Hospital,Hangzhou,310005,China [5]Department of Plastic and Reconstructive Surgery,Shanghai Ninth People’s Hospital,Shanghai Jiao Tong University,School of Medicine,Shanghai,200011,China

出　　处：《Regenerative Biomaterials》2025年第1期32-46,共15页再生生物材料(英文版)

基　　金：Funded by National Natural Science Foundation of China(82172076);Key Scientific Program of Jiangsu Provincial Health Commission(ZD2021033);Gusu Health Leading Talent Program of Suzhou(GSWS2021020);Discipline construction project of the Second Affiliated Hospital of Soochow University(XKTJXK202004);Scientific Program of Suzhou Municipal Health and Health Committee(LCZX202004);Project of Medical New Technology Assistance of the Second Affiliated Hospital of Soochow University(23ZL004 and 23ZL012).

摘　　要：Cartilage tissue engineering offers hope for tracheal cartilage defect repair.Establishing an anti-inflammatory microenvironment stands as a prerequisite for successful tracheal cartilage restoration,especially in immunocompetent animals.Hence,scaffolds inducing an anti-inflammatory response before chondrogenesis are crucial for effectively addressing tracheal cartilage defects.Herein,we develop a shell–core structured PLGA@ICA-GT@KGN nanofilm using poly(lactic-co-glycolic acid)(PLGA)and icariin(ICA,an antiinflammatory drug)as the shell layer and gelatin(GT)and kartogenin(KGN,a chondrogenic factor)as the core via coaxial electrospinning technology.The resultant PLGA@ICA-GT@KGN nanofilm exhibited a characteristic fibrous structure and demonstrated high biocompatibility.Notably,it showcased sustained release characteristics,releasing ICA within the initial 0 to 15days and gradually releasing KGN between 11 and 29days.Subsequent in vitro analysis revealed the potent anti-inflammatory capabilities of the released ICA from the shell layer,while the KGN released from the core layer effectively induced chondrogenic differentiation of bone marrow stem cells(BMSCs).Following this,the synthesized PLGA@ICA-GT@KGN nanofilms were loaded with BMSCs and stacked layer by layer,adhering to a‘sandwich model’to form a composite sandwich construct.This construct was then utilized to repair circular tracheal defects in a rabbit model.The sequential release of ICA and KGN facilitated by the PLGA@ICA-GT@KGN nanofilm established an anti-inflammatory microenvironment before initiating chondrogenic induction,leading to effective tracheal cartilage restoration.This study underscores the significance of shell–core structured nanofilms in temporally regulating anti-inflammation and chondrogenesis.This approach offers a novel perspective for addressing tracheal cartilage defects,potentially revolutionizing their treatment methodologies.

关 键 词：temporal control core-shell structured NANOFILM tracheal cartilage regeneration ANTI-INFLAMMATION 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]