Injectable cartilage microtissues based on 3D cultureusing porous gelatin microcarriers for cartilagedefect treatment  

作　　者：Jing Zhu Qiuchen Luo Tiefeng Cao Guang Yang Lin Xiao 

机构地区：[1]School of Biomedical Engineering,Shenzhen Campus of Sun Yat-Sen University,Shenzhen 518107,China [2]Department of Gynaecology,First Affiliated Hospital of Sun YatSen University,Guangzhou 510070,China [3]Department of Biomedical Engineering,College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China

出　　处：《Regenerative Biomaterials》2025年第1期72-83,共12页再生生物材料(英文版)

基　　金：supported by the National Natural Science Foundation of China(52173151,82002734,51803067);the Natural Science Foundation of Guangdong Province of China(2021A1515011084,2019A1515110312);the Fundamental Research Funds for the Central Universities(22qntd1302);the Shenzhen Outbound Postdoctoral Scientific Research Fund(SZBH202108).

摘　　要：Cartilage tissues possess an extremely limited capacity for self-repair,and current clinical surgical approaches for treating articular cartilage defects can only provide short-term relief. Despite significantadvances in the field of cartilage tissue engineering, avoiding secondary damage caused by invasive surgical procedures remains achallenge. In this study, injectable cartilage microtissues were developed through 3D culture of rat bone marrow mesenchymal stemcells (BMSCs) within porous gelatin microcarriers (GMs) and induceddifferentiation. These microtissues were then injected for thepurpose of treating cartilage defects in vivo, via a minimally invasiveapproach. GMs were found to be noncytotoxic and favorable for cellattachment, proliferation and migration evaluated with BMSCs. Moreover, cartilage microtissues with a considerable number of cells andabundant extracellular matrix components were obtained from BMSC-laden GMs after induction differentiation culture for 28days. Notably,ATDC5 cells were complementally tested to verify that the GMs were conducive to cell attachment, proliferation, migration and chondrogenicdifferentiation. The microtissues obtained from BMSC-laden GMs were then injected into articular cartilage defect areas in rats and achievedsuperior performance in alleviating inflammation and repairing cartilage. These findings suggest that the use of injectable cartilagemicrotissues in this study may hold promise for enhancing the long-term outcomes of cartilage defect treatments while minimizing the risk ofsecondary damage associated with traditional surgical techniques.

关 键 词：cartilage repair microtissue engineering MICROCARRIERS GELATIN BMSCS 

分 类 号：R31[医药卫生—基础医学]