莫海威芽孢杆菌JK-18的全基因组测序及拮抗相关基因簇的分析  

Genome Sequencing and Antimicrobial Metabolite Synthesis Genes of Bacillus mojavensis JK-18

作　　者：王瀚 王攀 田凤鸣 卓平清 马静静 WANG Han;WANG Pan;TIAN Fengming;ZHUO Pingqing;MA Jingjing(College of Agriculture and Forestry,Longnan Normal University,Longnan,Gansu 742500,China;College of Mechanical and Electrical Engineering,Gansu Agricultural University,Lanzhou,Gansu 730070,China)

机构地区：[1]陇南师范学院农林技术学院,甘肃陇南742500 [2]甘肃农业大学机电工程学院,甘肃兰州730070

出　　处：《福建农业学报》2025年第1期81-90,共10页Fujian Journal of Agricultural Sciences

基　　金：陇南市科技计划项目(2022-S.JH-12);甘肃省科学技术协会2023年创新驱动项目(GXH20230817-8)。

摘　　要：【目的】对核桃黑斑病病原拮抗菌莫海威芽孢杆菌JK-18进行全基因组测序和基因功能注释,初筛多种活性代谢产物合成基因簇。【方法】采用PacBio测序技术对从核桃根际土壤中筛选得到的莫海威芽孢杆菌JK-18进行全基因组测序,获得菌株基因组特征信息、基因功能注释及分类和次级代谢产物等关键信息。【结果】JK-18的基因组为一条环状闭合DNA,大小为4166746 bp,由1个环状染色体组成,共有4138个蛋白质编码基因。基因组中共预测到4种类型的CRISPR。GO、KEGG、EGGNOG、CAZY和CARD数据库中分别注释到5844、2299、3257、183和7个功能基因。应用在线软件AntiSMASH预测,该菌株基因组中包含Lichenysin、Macrobrevin和Aurantinin等聚酮类,Surfactin、Bacillomycin、Plipastatin、Fengycin等脂肽类,Dumulmycin及Dactylocycline A大环内酯类等多种活性代谢产物合成基因簇。【结论】通过全基因组测序及相关功能基因的挖掘分析,表明该菌株具有较好的生防潜能。相关研究结果对抗菌物质的后期深入研究具有重要的参考价值。【Objective】Sequence and annotation of Bacillus mojavensis JK-18 genome were obtained,and gene clusters related to the synthesis of antimicrobial secondary metabolites in the bacterium identified for the development of a biocontrol agent for walnut tree blight.【Method】The whole genome of B.mojavensis JK-18 isolated from the rhizosphere soil of walnut trees was sequenced using the PacBio technology.The characteristics,functions,and classification of the gene and secondary metabolites secreted by the bacterium were analyzed.【Results】The genome was a closed circular DNA with a size of 4166746 bp containing 4138 protein-coding genes and 4 kinds of CRISPR.It had 5844,2299,3257,183,and 7 functional genes annotated in GO,KEGG,EGGNOG,CAZY,and CARD databases,respectively.Predicted by antiSMASH,the genes related to the synthesis of secondary metabolites including the antimicrobial substances like polyketide compounds(e.g.,Lichenysin,Macrobrevin and Aurantinin),peptidolipids(e.g.,Surfactin,Bacillomycin,Plipastatin and Fengycin),and macrolides(e.g.,Dumulmycin and Dactylocycline A).【Conclusion】Identification of the genes in B.mojavensis JK-18 that related to the syntheses of secondary metabolites with an antimicrobial property would aid further study leading to the development of bioagents for blight prevention and control on walnut trees.

关 键 词：核桃 莫海威芽孢杆菌 全基因组测序 合成基因簇 

分 类 号：S436[农业科学—农业昆虫与害虫防治]