核黄素通过抑制缺血性脑卒中小鼠神经元凋亡缩小梗死范围  

Riboflavin reduces the range of ischemic stroke infarction by inhibiting the neuronal apoptosis in mice

作　　者：杨维 庞娟 夏宇航 李俊 杨翰 尚粉青 殷俊茹 YANG Wei;PANG Juan;XIA Yuhang;LI Jun;YANG Han;SHANG Fenqing;YIN Junru(Translational Medicine Centre,Xi’an Chest Hospital,Xi’an 710100,China;Department of Cardiovascular Medicine,Xi’an Chest Hospital,Xi’an 710100,China;Department of Anesthesiology,Xi’an Chest Hospital,Xi’an 710100,China)

机构地区：[1]西安市胸科医院转化医学中心,西安710100 [2]西安市胸科医院心血管内科,西安710100 [3]西安市胸科医院麻醉科,西安710100

出　　处：《神经解剖学杂志》2025年第1期25-31,共7页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82370406);陕西省自然科学基础研究计划(2023-JC-YB-799);西安市卫生健康委员会科研项目(2023ms16)。

摘　　要：目的:探讨核黄素对小鼠脑缺血损伤后脑组织梗死体积的影响及其抑制神经元凋亡的可能作用途径机制。方法:18只C57BL/6J雄性小鼠随机分为三组:假手术组(Sham)、大脑中动脉栓塞(MCAO)模型组、核黄素干预组(MCAO+RF);核黄素预处理1周后造模,24 h后经2,3,5-氯化三苯基四氮唑(TTC)染色观察各组小鼠脑组织梗死范围;采用实时荧光定量聚合酶链式反应(RT-qPCR)检测梗死侧脑组织中凋亡相关蛋白肿瘤蛋白p53(p53)、细胞色素C(CytC)、B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸蛋白酶-3(caspase-3)、多腺苷二磷酸核糖聚合酶(PARP)、半胱氨酸蛋白酶-6(caspase-6)、细胞凋亡诱导因子(AIF)的mRNA表达变化,筛查核黄素抑制神经元凋亡的可能的作用途径;Western blot检测乙酰化p53(AC-p53)、caspase-3、PARP的蛋白表达。结果:与MCAO模型组相比,MCAO+RF组脑梗死体积显著减少(P<0.01);核黄素的使用可抑制小鼠脑组织中p53、CytC、caspase-3、PARP、caspase-6、AIF的表达且差异显著(P<0.05);同时AC-p53、caspase-3、PARP的蛋白表达亦显著降低(P<0.05)。结论:核黄素可通过多途径抑制神经元的凋亡,改善缺血性脑卒中导致的大脑损伤。Objective:To investigate the effect of riboflavin on cerebral infarction volume and the possible mechanism of apoptotic factors with cerebral ischemic injury in mice.Methods:Eighteen C57BL/6J male mice were divided into the sham group,middle cerebral artery occlusion(MCAO)group and riboflavin intervention group(MCAO+RF)randomly.TTC staining was used to observe the infarction of the cerebral tissues;Quantitative realtime PCR(RT-qPCR)was used to detect the mRNA expression of tumor protein p53(p53),cytochrome C(CytC),B-cell lymphoma-2(Bcl-2),Bcl-2-associated X(Bax),cysteinyl aspartate specific proteinase-3(caspase-3),poly ADP-ribose polymerase(PARP),cysteinyl aspartate specific proteinase-6(caspase-6)and apoptosis inducing factor(AIF)in different groups,to study the possible mechanism of riboflavin inhibiting neuronal apoptosis.The proteins expression of acetyl-p53(AC-p53),caspase-3 and PARP were analyzed by Western blot.Results:Compared with the MCAO group,the cerebral infarct volume of the MCAO+RF group was obviously reduced(P<0.01);The relative expression of p53,CytC,caspase-3,PARP,caspase-6 and AIF were significantly lower in the MCAO+RF group(P<0.05).Additionally,significant differences were observed in the proteins expression of AC-p53,caspase3 and PARP between the MCAO group and MCAO+RF group.Conclusion:Riboflavin has a protective effect against cerebral ischemic injury,which is possibly realized by inhibiting neuronal apoptosis through multiple pathways.

关 键 词：核黄素 缺血性脑卒中 大脑中动脉栓塞 神经元凋亡 小鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学]