脑卒中后吞咽障碍小鼠模型中不同麻醉剂作用的比较  

Comparison of the actions of different anesthetics in the post-stroke dysphagia model of mouse

作　　者：饶彤 饶泽华 田聪 陆梦 陈安坤 刘欣 慕容志苗 岳增辉[1] RAO Tong;RAO Zehua;TIAN Cong;LU Meng;CHEN Ankun;LIU Xin;MURONG Zhimiao;YUE Zenghui(School of Acupuncture,Massage and Rehabilitation,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿与康复学院,长沙410208

出　　处：《神经解剖学杂志》2025年第1期59-66,共8页Chinese Journal of Neuroanatomy

基　　金：国家自然科学基金(82205298);湖南省自然科学基金面上项目(2023JJ30462);湖南省中医药管理局中医药科研一般项目(B2024007);湖南中医药大学校级科研项目(Z2023XJYQ07,Z2023YYJJ02);湖南中医药大学研究生创新课题(2024CX099)。

摘　　要：目的:探索3种麻醉剂(三溴乙醇、异氟烷、戊巴比妥钠)对光栓塞(PT)脑卒中后吞咽障碍(PSD)模型小鼠的影响。方法:将66只雄性C57BL/6J小鼠分为三溴乙醇(Tribromoethanol)组、异氟烷(Isoflurane)组、戊巴比妥钠(Pentobarbital sodium)组和假手术(Sham)组。使用PT法制备PSD模型。造模前、造模后5 min使用激光散斑成像仪测量小鼠的区域脑血流(rCBF)下降率并且记录小鼠的苏醒时间,造模后48 h记录3组PSD小鼠的死亡率、再次测量rCBF下降率。使用神经功能缺损评分评估小鼠的神经功能,使用4 min饮水试验记录小鼠的饮水量,使用TTC染色法测量梗死体积比率,使用多导生理记录仪MP160记录给水诱发的吞咽次数并且计算吞咽肌的肌电面积。结果:3组PSD小鼠在造模48 h后rCBF下降率、梗死体积比率、神经功能缺损评分、饮水量、吞咽次数、吞咽肌肌电面积不存在统计学差异(P>0.05)。相较于三溴乙醇组和戊巴比妥钠组,异氟烷组小鼠在5 min内rCBF迅速下降(P<0.05),并且小鼠的死亡率更低、苏醒时间更短(P<0.05)。结论:不同麻醉的使用会影响PSD小鼠的死亡率、苏醒时间和造模后48 h内rCBF下降趋势,这3种麻醉剂中异氟烷更适合作为PSD小鼠的麻醉剂。Objective:To explore the effects three of anesthetics(tribromoethanol,isoflurane,and pentobarbital sodium)on the outcome of mice with post-stroke dysphagia(PSD)induced by photothrombosis(PT)method,and to evaluate which anesthetic is more suitable for the preparation of this model.Methods:Sixty-six male C57BL/6J mice were divided into Tribromoethanol group,Isoflurane group,Pentobarbital sodium group and Sham group.The post-stroke dysphagia model was established by PT.Before and 5 min after modeling,a laser speckle imager was used to measure the regional cerebral blood flow(rCBF)decrease rate of mice and record the wake-up time of mice.Forty-eight hours after modeling,the mortality rate of PSD mice in three groups was recorded and the rCBF decrease rate was measured again.The neurological function of mice was evaluated using the neurological deficit score,the water intake of mice was recorded using the 4-min drinking test,the infarct volume ratio was measured using the TTC staining method,and the swallowing counts induced by water administration was recorded using the multichannel physiological recorder MP160 and the myoelectric area of the swallowing muscle was calculated.Results:There was no statistical difference in the percentage of decrease in rCBF,infarct volume ratio,neurological deficit score,water intake,swallowing counts,and myoelectric area of swallowing muscle among the three groups of PSD mice 48 h after modeling(P>0.05).Compared with the Tribromoethanol group and the Pentobarbital sodium group,the rCBF of the mice in the Isoflurane group decreased rapidly within 5 min(P<0.05),and the mortality rate of the mice was lower and the awakening time was shorter.(P<0.05).Conclusion:The use of different anesthesia will affect the mortality rate,wake-up time and the downward trend of rCBF within 48 h after modeling of PSD mice.Among the three anesthetics,isoflurane is more suitable as an anesthetic for the PSD mouse model.

关 键 词：异氟烷 戊巴比妥钠 三溴乙醇 光栓塞 脑卒中后吞咽障碍 吞咽功能 小鼠 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]