10种百合鳞茎多酚类提取物抗氧化活性及其与环境因子相关性  

Antioxidant Activity of Polyphenol Extracts from Ten Lily Bulbs and Its Correlation with Environmental Factors

作　　者：马祥臣 张萍[1] 陈雨欣 靳磊 MA Xiangchen;ZHANG Ping;CHEN Yuxin;JIN Lei(College of Forestry and Prataculture,Ningxia University,Yinchuan 750021)

机构地区：[1]宁夏大学林业与草业学院,银川750021

出　　处：《植物研究》2025年第2期315-328,共14页Bulletin of Botanical Research

基　　金：国家自然科学基金项目(32260727);宁夏自治区重点研发项目(2021BBF02025)。

摘　　要：该研究以采集自30个地点的10种百合(Lilium)为材料,采用比色法测定百合鳞茎的多酚类物质含量及抗氧化活性,采用凯氏定氮法和原子吸收法测定百合鳞茎和采集地土壤的氮、磷、钾含量,并调查采集地环境条件,旨在探索野生百合在抗氧化功能性食品开发方面的应用前景,为进一步开发百合资源提供参考。结果表明:采集自30个地点的10种百合鳞茎中多酚、黄酮、总黄烷醇和总花色苷含量存在显著差异(P<0.05);不同采集地同种百合鳞茎中多酚类物质含量存在显著差异(P<0.05),表明百合种源的品质存在明显的生态区域差异。10种百合鳞茎多酚类提取物对1,1-二苯基-2-三硝基苯肼(DPPH)自由基清除能力、铜离子还原能力、金属螯合能力和抑制脂质过氧化能力存在显著差异(P<0.05),且野生百合鳞茎多酚类提取物的抗氧化活性强于传统食用的兰州百合(L.davidii var.unicolor);不同采集地同种百合鳞茎多酚类提取物抗氧化活性存在显著差异(P<0.05),表明百合种源的抗氧化活性具有明显的“道地性”。10种百合鳞茎氮、磷、钾含量存在显著差异(P<0.05),采集地土壤中的氮、磷、钾含量存在显著差异(P<0.05)。相关性分析表明,10种百合鳞茎的多酚类物质含量与铜离子还原能力、DPPH自由基清除能力和抑制脂质过氧化能力之间存在显著或极显著相关性;百合鳞茎多酚类物质含量及其抗氧化活性与环境因子之间存在不同程度的相关性;采集地环境因子对百合鳞茎中多酚类物质含量及其抗氧化活性产生不同影响,总体以年降水量、年均温度为主导,多酚含量与年最低温度、年降水量和年均温度之间呈显著正相关(P<0.05);总花色苷含量与年均温度和风速呈显著正相关(P<0.05)。利用隶属函数对百合多酚抗氧化活性进行综合评价,结果表明:野生百合鳞茎的抗氧化活性优于传统食用的兰州百合,其中四�In this study,10 lily species collected from 30 areas were used as materials to determine the polyphenols content and antioxidant activity in lily bulbs by colorimetry,and the contents of nitrogen,phosphorus and potassium in lily bulbs and soils were determined by Kjeldahl method and atomic absorption spectrometry method,respectively.The environmental conditions of collection areas were investigated to explore the application prospect of wild lily in the development of antioxidant functional foods.This work provided the reference for further development of lily resources.The results showed that the contents of polyphenols,flavonoids,total flavanols and total anthocyanins in 10 lily bulbs collected from 30 areas were significantly different(P<0.05).The content of polyphenols in bulbs of the same lily species collected from different areas were significantly different(P<0.05),indicating the differences in lily quality of provenances among the ecological regions.There were significant differences in DPPH radical scavenging ability,copper ion reduction ability,metal chelation ability and lipid peroxidation inhibition ability of 10 lily bulb extracts(P<0.05),and the antioxidant ability of polyphenol extracts from wild lily bulb was better than that from cultivated Lilium davidii var.unicolor.The antioxidant activity of polyphenol extracts from the same lily bulbs collected from different areas were significantly different(P<0.05),which indicated that the antioxidant capacity of lily origin was obviously“authentic”.The contents of N,P and K in 10 lily bulbs were significantly different(P<0.05),and the contents of N,P and K in soils collected from collection areas were significantly different(P<0.05).Correlation analysis showed significant or extremely significant correlations between polyphenols content and copper ion reduction ability,DPPH free radical scavenging ability and lipid peroxidation inhibition ability of lily bulbs.Polyphenol content and antioxidant capacity of 10 lily bulbs were correlated with environ

关 键 词：百合 鳞茎 多酚 抗氧化 环境因子 

分 类 号：Q945[生物学—植物学]