开放性实验:QuEChERS联合荧光衍生法检测莠去津及其对酶活性的影响  

Open experiment:QuEChERS combined with fluorescence derivatization for the detection of atrazine and its effect on enzyme activity

作　　者：黄兴华 黄译瑶 高武 张一达 刘晓燕[1] 张海霞[1] HUANG Xinghua;HUANG Yiyao;GAO Wu;ZHANG Yida;LIU Xiaoyan;ZHANG Haixia(College of Chemistry and Chemical Engineering,Lanzhou University,Lanzhou 730000,China)

机构地区：[1]兰州大学化学化工学院,甘肃兰州7300000

出　　处：《色谱》2025年第4期388-393,共6页Chinese Journal of Chromatography

基　　金：兰州大学双语教学项目;兰州大学课程思政项目;兰州大学课程认证项目.

摘　　要：莠去津是三嗪类农药的代表,其不仅能干扰生物正常生理活动,还可通过诱发氧化应激对植物体造成明显的毒性作用。因此,莠去津的定量检测和毒性机理研究对保证食品安全和维持植物正常生长十分重要。本实验建立了一种QuEChERS和荧光衍生结合的策略,实现了实际样品中莠去津的快速灵敏定量检测。该策略具有选择性好、灵敏度高和操作简单的优点。此外,通过酶活性考察与分子对接探究了莠去津对过氧化氢酶活性的影响,实现了莠去津的毒性机理探究和分子层面验证。通过两部分工作的有机结合,回答了“为什么测”“怎么测”“原理是什么”的问题。本实验涉及样品前处理、光谱和分子对接的相关知识,使用的仪器在高校中普及程度较高,十分适合作为开放性实验进行实验教学。同时,本实验力图通过全方位实验操作、理论结合和应用研究,使学生充分了解分离材料、荧光衍生试剂的选择与应用、分析仪器的检测原理、酶的使用和分子对接相关原理及应用范围。该实验不仅有助于增强学生关注民生、注重分析化学理念和操作规范意识,还能指导学生初步掌握科学研究的思维方法并提升解决实际问题的综合能力。Atrazine is a triazine pesticide that interferes with normal physiological activities,induces oxidative stress,and is significantly toxic to plants.Therefore,the ability to quantitatively detect atrazine and study the mechanisms responsible for its toxicity are crucial for ensuring food safety and maintaining plant growth.Herein,a method was developed by QuEChERS combined with fluorescence derivatization to rapidly,sensitively,and quantitatively detect atrazine in real samples.This approach offers excellent selectivity and high sensitivity,and is simple to operate.In addition,we also investigated the effect of atrazine on catalase activity through enzyme-activity assays and molecular-docking studies to explore the mechanism responsible for the toxicity of atrazine on the molecular level.This experiment involves knowledge related to sample pretreatment,spectroscopy,and molecular docking,while the instruments used are widely available in colleges and universities and are very suitable for teaching open experiments.This experiment aims to provide students with a comprehensive understanding of experimental operations integrated with theory,as well as applied research aimed at helping students understand the use of separation materials,the selection and application of fluorescent derivatization reagents,the principles of analytical instruments,the use of enzymes,and the principles of molecular docking.This experiment is expected to enhance students’awareness of people’s livelihoods,analytical chemistry concepts,and operational standards,which will guide the development of their scientific research thinking and improve their abilities to solve real-world problems.

关 键 词：开放性实验 QUECHERS 荧光检测 莠去津 过氧化氢酶 分子对接 

分 类 号：O658[理学—分析化学]