血管腔内介入技术用于构建肝静脉阻塞型巴德-基亚里综合征犬模型  

Endovascular intervention technology for constructing canine models of hepatic vein obstruction type Budd-Chiari syndrome

作　　者：孙学栋 段峰 马超 宋振飞 王茂强 金龙[1] SUN Xuedong;DUAN Feng;MA Chao;SONG Zhenfei;WANG Maoqiang;JIN Long(Department of Interventional Radiology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Interventional Radiology,the Fifth Medical Center,Chinese PLA General Hospital,Beijing 100853,China)

机构地区：[1]首都医科大学附属北京友谊医院放射介入科,北京100050 [2]中国人民解放军总医院第五医学中心介入放射科,北京100853

出　　处：《中国介入影像与治疗学》2025年第3期205-209,共5页Chinese Journal of Interventional Imaging and Therapy

摘　　要：目的评估血管腔内介入技术用于建立肝静脉(HV)阻塞型巴德-基亚里综合征(BCS)犬模型的可行性。方法对10只比格犬经颈外静脉及经皮经肝穿刺HV双入路途径以双球囊阻断肝静脉主干后注入无水乙醇,构建HV阻塞型BCS模型,对比造模前、后实验室指标。分别于造模后2、4、8周行肝脏超声,末次超声后行肝静脉血管腔内超声(IVUS)和数字减影血管造影(DSA),观察肝左静脉(LHV)阻塞程度、管腔及血流,基于DSA所见评估LHV主干狭窄率,以狭窄率>30%而未完全闭塞为造模成功;记录造模成功率。结果造模前、后实验室各项指标差异均无统计学意义(P均>0.05)。造模后超声、IVUS和DSA显示9只犬LHV管腔不同程度节段性狭窄、伴肝内交通静脉侧支循环建立,1只犬出现LHV弥漫性完全闭塞;造模成功率为90.00%(9/10)。病理结果显示靶HV管腔狭窄、管壁内膜增厚,后者主要成分为增生成纤维细胞和胶原纤维。结论血管腔内介入技术可用于构建HV阻塞型BCS犬模型。Objective To explore the feasibility of endovascular intervention technology for constructing canine models of hepatic vein(HV)obstruction type Budd-Chiari syndrome(BCS).Methods HV obstruction type BCS models were established through double approaches including external jugular vein and percutaneous transhepatic vein puncture,blocking the main HV with double balloon and injecting anhydrous ethanol using 10 Beagle dogs.Laboratory indexes before and after modeling were compared.Liver ultrasound was performed 2,4 and 8 weeks after modeling,respectively,while intravascular ultrasound(IVUS)and digital subtracting angiography(DSA)were performed after the last time ultrasound to observe the obstruction degree,lumen and blood flow of left hepatic vein(LHV).The stenosis rate of LHV was evaluated based on DSA findings,and successful establishment of HV obstruction type BCS canine model was regarded as stenosis rate>30%and incomplete occlusion of LHV,then the success rate of modeling was recorded.Results No significant difference of laboratory indexes was found before and after modeling(all P>0.05).Ultrasound,IVUS and DSA showed that after modeling,different degrees of LHV stenosis complicated with intrahepatic collateral circulation were found in 9 dogs,while complete occlusion of LHV was noticed in 1 dog.The success rate of modeling was 90.00%(9/10).Pathological results showed the target HV lumen narrow with thickened endovascular wall,and the latter mainly composed of fibroblast and collagen fiber proliferation.Conclusion Endovascular intervention technology was feasible for constructing canine models of HV obstruction type BCS.

关 键 词：巴德-基亚里综合征 肝静脉阻塞症 犬 模型 动物 

分 类 号：R575[医药卫生—消化系统] R-332[医药卫生—内科学]