桦木汁对斑马鱼胚胎的体外抗氧化作用及体内酶学检测  

Antioxidant effects and enzymatic analysis of birch sap on zebrafish embryos

作　　者：邢琦 陈海燕 华李羽[2] 王超 李轲 刘纪盈 XING Qi;CHEN Haiyan;HUA Liyu;WANG Chao;LI Ke;LIU Jiying(Dezhou Huashang Refrigerated Logistics Co.,Ltd.,Dezhou,Shandong 253000,China;School of Life Sciences,Changchun University of Science and Technology,Changchun,Jilin 130600,China;School of Pharmacy,Changchun University of Traditional Chinese Medicine,Changchun,Jilin 130000,China;School of Food Science and Engineering,Jilin Agricultural University,Changchun,Jilin 130118,China)

机构地区：[1]德州华商冷藏物流有限公司,山东德州253000 [2]长春科技学院生命科学学院,吉林长春130600 [3]长春中医药大学药学院,吉林长春130000 [4]吉林农业大学食品科学与工程学院,吉林长春130118

出　　处：《食品与机械》2025年第2期160-165,共6页Food and Machinery

基　　金：吉林省教育厅科学技术研究项目(编号:JJKH20241707CY)。

摘　　要：[目的]研究长白山天然桦木汁的抗氧化活性。[方法]通过DPPH自由基清除率、ABTS阳离子自由基清除率、羟自由基清除率、铁离子还原能力(FRAP)4种化学法测定其抗氧化活性水平,并利用模式生物—斑马鱼胚胎检测其动态酶学指标。[结果]桦木汁的抗氧化效果随汁液浓度的增加呈正相关,氧化活性因子显著。当桦木汁用量为1.0 mL/mL时,其抗氧化效果最佳,且对DPPH自由基清除率、ABTS阳离子自由基清除率、羟自由基清除率、铁离子还原能力分别为34.56%,18.27%,48.77%,90.07μmol/L,斑马鱼胚胎SOD、CAT、GSH-Px活性以及MDA含量相较于空白对照组均有显著提升。[结论]天然桦木汁具有良好的抗氧化作用。[Objective]This study aimed to evaluate the antioxidant activity of natural birch juice from Changbai Mountain.[Methods]Antioxidant activity was measured through four chemical assays:DPPH radical scavenging rate,ABTS cation scavenging rate,hydroxyl radical scavenging rate,ferric ion reducing ability(FRAP).Additionally,antioxidant enzyme activities and oxidative stress markers were assessed using zebrafish embryo as a model organism.[Results]The antioxidant activity of birch juice increased with concentration,showing a significant positive correlation.At 1.0 mL/mL concentration,birch juice exhibited optimal effects,achieving 34.56%DPPH scavenging,18.27%ABTS scavenging,48.77%hydroxyl radical scavenging,and 90.07μmol/L ferrous ion reduction.In zebrafish embryos,the activities of SOD,CAT,and GSH-Px were significantly elevated,along with increased MDA content,compared to the control group.[Conclusion]Natural birch juice demonstrates substantial antioxidant properties,highlighting its potential for development into natural antioxidant foods,as well as anti-aging and skin-whitening products.

关 键 词：桦木汁 斑马鱼胚胎 抗氧化性 自由基清除率 活性测定 

分 类 号：R28[医药卫生—中药学]