啤酒废酵母水解液养殖裂壶藻生产DHA藻油  

Production of DHA algal oil from Schizochytrium sp.fed with waste beer yeast hydrolysate

作　　者：杨庆懿 唐裕芳[2] 李涛 刘磊[2] 周大科 YANG Qingyi;TANG Yufang;LI Tao;LIU Lei;ZHOU Dake(Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning of Hunan Institute of Commodity Quality Inspection,Changsha,Hunan 410007,China;College of Chemical Engineering of Xiangtan University,Xiangtan,Hunan 411105,China;Hunan Zhujiang Beer Co.,Ltd.,Xiangtan,Hunan 411228,China)

机构地区：[1]湖南省产商品质量检验研究院食品安全监测与预警湖南省重点实验室,湖南长沙410007 [2]湘潭大学化工学院,湖南湘潭411105 [3]湖南珠江啤酒有限公司,湖南湘潭411228

出　　处：《食品与机械》2025年第2期173-180,共8页Food and Machinery

基　　金：湖南省自然科学基金面上项目(编号:2024JJ7545,2022JJ90027,2021JJ30660)。

摘　　要：[目的]探索啤酒废酵母水解液养殖裂壶藻生产DHA藻油的可行性。[方法]以还原糖质量浓度和总氮质量浓度为响应值优化超声辅助复合酶法破壁水解废啤酒酵母,并利用水解液培养裂壶藻生产DHA藻油。[结果]在超声功率400 W,温度60℃,pH 5.0,木瓜蛋白酶添加量0.5 g,m_(β-葡聚糖酶):m_(木瓜蛋白酶)=4:5,超声40 min,所得水解液中还原糖和总氮质量浓度达到最大,分别为20.40,6.72 g/L。水解液体积分数75%,裂壶藻接种体积分数14%,培养3d,裂壶藻的生物量、油脂产量以及DHA质量分数和产量达到最大,分别为17.14 g/L,2.88 g/L,3.13%,0.55 g/L,均较传统发酵培养养殖裂壶藻的生物量(10.29 g/L)、油脂产量(1.84 g/L)以及DHA质量分数(2.76%)和产量(0.29 g/L)高,所得藻油的致病菌和重金属指标均低于食品安全国家标准的最低限量。[结论]利用超声辅助复合酶法所得废啤酒酵母水解液养殖裂壶藻生产DHA是可行的,可以实现食品生产废弃物处理和微藻化工产品生产的有机统一。[Objective]To explore the feasibility of cultivating Schizochytrium sp.using waste beer yeast hydrolysate to produce DHA algal oil.[Methods]Ultrasound-assisted composite enzyme method was selected to break the cell wall and hydrolyze waste beer yeast,optimizing the process based on reducing sugar and total nitrogen concentrations as response variables.The hydrolysate was then used to cultivate Schizochytrium sp.to produce DHA algal oil.[Results]Under the conditions of ultrasonic power at 400 W,temperature at 60℃,pH 5.0,papain at 0.5 g,β-glucanase:papain ratio of 4:5,and hydrolysis time of 40 min,the hydrolysate achieved maximum concentrations of reducing sugar and total nitrogen,which were 20.40 g/L and 6.72 g/L,respectively.When the hydrolysate volume fraction was 75%and the inoculum volume fraction of Schizochytrium sp.was 14%,after 3 days of cultivation,the biomass,oil yield,DHA content,and DHA yield reached their maximum values of 17.14 g/L,2.88 g/L,3.13%,and 0.55 g/L,respectively,which were higher than those obtained from traditional fermentation cultures(biomass 10.29 g/L,oil yield 1.84 g/L,DHA content 2.76%,DHA yield 0.29 g/L).The pathogenic bacteria and heavy metal levels in the algal oil were below the minimum limits specified in national food safety standard.[Conclusion]Using waste beer yeast hydrolysate obtained through ultrasound-assisted composite enzyme hydrolysis to cultivate Schizochytrium sp.for DHA production is feasible.This method achieves an organic integration of food production waste treatment and microalgae-based chemical product production.

关 键 词：废啤酒酵母 水解液 裂壶藻 DHA 

分 类 号：TS2[轻工技术与工程—食品科学与工程]