白藜芦醇对高强度运动大鼠肾脏纤维化的影响及机制研究  

Study on the effect and mechanism of resveratrol on rats in renal fibrosis with high-intensity exercise

作　　者：周海涛[1] 牛衍龙 张静[3] 周绮云[3] 秦菲[3] ZHOU Hai-tao;NIU Yan-long;ZHANG Jing;ZHOU Qi-yun;QIN Fei(Institute of Physical Exercise and Health,Beijing Union University,Beijing 100101 China;School of Sport and Health,Guangzhou Sport University,Guangzhou 510500,China;College of Biochemical Engineering,Beijing Union University,Beijing 100023,China)

机构地区：[1]北京联合大学体育运动与健康研究所,北京100101 [2]广州体育学院运动与健康学院,广州510500 [3]北京联合大学生物化学工程学院,北京100023

出　　处：《天然产物研究与开发》2025年第3期411-420,共10页Natural Product Research and Development

基　　金：北京市教育委员会科技计划(KM202211417004)。

摘　　要：本文探究了白藜芦醇对高强度运动大鼠肾脏纤维化的影响及机制。雄性SD大鼠随机分为对照组(control group,CONT)、白藜芦醇组(resveratrol group,RESV)、运动性肾损伤模型组(exercise-induced kidney injury model group,EIKIM)和白藜芦醇干预的运动性肾损伤模型组(resveratrol-intervened exercise-induced kidney injury model group,REIKIM)。末次训练结束24 h后采集血液和肾脏组织样本,评估大鼠肾脏组织形态及纤维化水平,并检测相关生化指标。结果表明,相较于CONT组大鼠,EIKIM组大鼠肾脏组织出现明显的病理学改变和纤维化;血清中肌酐(creatinine,Cr)和尿素氮(urea nitrogen,UN)水平、肾脏组织胶原沉积评分及磷酸化p38丝裂原活化蛋白激酶(phosphorylated p38 mitogen-activated protein kinase,p-p38 MAPK)、核转录因子κB(nuclear factor kappa-light-chain-enhancer of activated B cells,NF-κB)和乙酰化核转录因子κB(acetylated nuclear factor kappa-light-chain-enhancer of activated B cells,Ac-NF-κB)蛋白质表达水平和组织转化生长因子β1(transforming growth factorβ1,TGF-β1)、肿瘤坏死因子α(tumor necrosis factorα,TNF-α)、白细胞介素-1β(interleukin-1β,IL-1β)水平显著升高(P<0.01);沉默信息调节因子1(silent information regulator 1,SIRT1)蛋白质表达水平显著降低(P<0.01)。相较于EIKIM组大鼠,REIKIM组大鼠肾脏组织的病理学改变及纤维化改善明显;血清中Cr和UN水平、肾脏组织胶原沉积评分及p-p38 MAPK、NF-κB、Ac-NF-κB蛋白质表达水平和TGF-β1、TNF-α、IL-1β水平显著降低(P<0.05,P<0.01);SIRT1蛋白质表达水平显著升高(P<0.01)。综上所述,白藜芦醇通过调控p38 MAPK/SIRT1/NF-κB信号通路关键蛋白质表达,有效抑制了高强度运动诱发的大鼠肾脏炎症反应,发挥了减轻肾脏纤维化的发展、保护肾脏结构与功能的作用。This study investigated the effect and mechanism of resveratrol on rats in renal fibrosis with high-intensity exercise.Male SD rats were randomly divided into control group(CONT),resveratrol group(RESV),exercise-induced kidney injury model group(EIKIM),and resveratrol-intervened exercise-induced kidney injury model group(REIKIM).Specimens of blood and renal tissues were harvested 24 h after the final training to evaluate the histomorphology and extent of fibrosis in the rat kidneys,in addition to examining pertinent biochemical parameters.The findings revealed that,compared to CONT group,EIKIM group exhibited significant pathological changes and fibrosis in renal tissues.Serum creatinine(Cr)and urea nitrogen(UN),as well as renal collagen deposition scores,protein expression levels of phosphorylated p38 mitogen-activated protein kinase(p-p38 MAPK),nuclear factor kappa-light-chain-enhancer of activated B cells(NF-κB),acetylated nuclear factor kappa-light-chain-enhancer of activated B cells(Ac-NF-κB),transforming growth factor beta-1(TGF-β1),tumor necrosis factor alpha(TNF-α),and interleukin-1 beta(IL-1β)levels were markedly increased(P<0.01).Conversely,the protein expression level of silent information regulator 1(SIRT1)was significantly decreased(P<0.01).Compared to EIKIM group,REIKIM group demonstrated a marked improvement in pathological changes and fibrosis in renal tissue.Notably,serum levels of Cr and UN,as well as renal collagen deposition scores,protein expression of p-p38 MAPK,NF-κB,Ac-NF-κB,and TGF-β1,TNF-α,IL-1βlevels were significantly reduced(P<0.05,P<0.01).Conversely,the SIRT1 expression was significantly elevated(P<0.01).In summary,resveratrol effectively suppressed the renal inflammatory response induced by high-intensity exercise in rats by modulating the expression of key proteins in p38 MAPK/SIRT1/NF-κB signaling pathway,thereby mitigating the progression of renal fibrosis and preserving renal structure and function.

关 键 词：白藜芦醇 高强度运动大鼠 肾脏纤维化 炎症反应 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]