鸡柔嫩艾美耳球虫EtIMP1基因的克隆与表达载体的构建  

Cloning and construction of expression vector for EtIMP1 gene of chicken Eimeria tenella

作　　者：廖加磊 Liao Jialei(Agricultural and Rural Bureau of Yanping District,Nanping City,Fujian Nanping353000)

机构地区：[1]南平市延平区农业农村局,福建南平353000

出　　处：《福建畜牧兽医》2025年第2期54-56,共3页Fujian Journal of Animal Husbandry and Veterinary medicine

摘　　要：本研究对鸡柔嫩艾美耳球虫RNA进行提取及反转录,克隆鸡柔嫩艾美耳球虫IMP1基因,并将PCR产物连接于pEasy-Blunt-Simple载体中,PCR鉴定正确后送公司测序,进行序列分析。结果:成功克隆鸡柔嫩艾美耳球虫EtIMP1基因,其ORF长度为1 194 bp,并验证了其正确性;构建鸡柔嫩艾美耳球虫EtIMP1基因表达载体pEasy-Blunt-Simple-IMP1,并得到重组质粒,可为体外表达IMP1基因提供技术方法,也为疫苗候选基因的筛选奠定基础。This study extracted and reverse transcribed RNA from chicken Eimeria tenella,cloned the IMP1 gene,and connect the PCR product to the pEasy-Blunt-Simple vector,identify it correctly by PCR,and send it to the company for sequencing and sequence analysis.Result:The EtIMP1 gene of chicken Eimeria tenella was successfully cloned,with an ORF length of 1194 bp,and its correctness was verified;Constructing the EtIMP1 gene expression vector pEasy-Blunt-Simple IMP1 and obtaining a recombinant plasmid,which can provide a technical method for in vitro expression of the IMP1 gene and lay the foundation for screening vaccine candidate genes.

关 键 词：鸡柔嫩艾美耳球虫 IMP1基因 克隆 表达载体 PCR 

分 类 号：R73[医药卫生—肿瘤]