广西三黄鸡胚胎源多重耐药肠炎沙门氏菌分离鉴定及抗菌药物筛选  

Isolation and antimicrobial screening of multidrug resistant Salmonella enterica from the embryos of Guangxi Sanhuang chicken

作　　者：黄华汉 Huang Huahan(Debao Horse Culture Industry Development Center,Guangxi Debao533700)

机构地区：[1]德保县矮马文化产业发展中心,广西德保533700

出　　处：《福建畜牧兽医》2025年第2期61-63,共3页Fujian Journal of Animal Husbandry and Veterinary medicine

摘　　要：通过细菌分离纯化、生化试验、血清型鉴定、16S r RNA扩增等方法,从广西三黄鸡胚胎中分离到1株肠炎沙门氏菌。药敏试验显示分离菌株对阿米卡星、环丙沙星、氧氟沙星、头孢噻呋钠等药物敏感,对庆大霉素、洛美沙星、阿莫西林等耐受;接种4周龄昆明小鼠,致死率为100%,说明该菌株是一株致病性强的多重耐药菌。临床中可以使用阿米卡星、头孢噻呋钠等药物进行防控。本研究可为提升地方品种鸡孵化率提供科学参考。A series of tests,including bacterial isolation,morphological characteristics,serotype analysis,16S rRNA gene sequencing analysis,the drug sensitivity and pathogenicity of the strain were performed on the embryos of Guangxi Sanhuang chicken,a strain of Salmonella enterica was isolated.The results of drug sensitivity test demonstrated that the isolated strain was sensitive to amikacin,ciprofloxacin,ofloxacin and ceftiofur sodium,resistant to gentamycin,lomefloxacin,amoxicillin.The mortality rate of Kunming mice was 100%.So,the strain showed multiple drug-resistance phenotype and high virulence.Amikacin and ceftiofur sodium could be used to prevent Salmonella enterica in the subsequent work.This study provided the scientific reference for the promotion of the hatchability of local breed chickens.

关 键 词：地方鸡 胚胎源 多重耐药 肠炎沙门氏菌 

分 类 号：S85[农业科学—兽医学]