Features of the monocyte inflammatory response in patients with premature coronary artery disease  

作　　者：Tatiana Blokhina Tatiana Kirichenko Yuliya Markina Ulyana Khovantseva Ivan Melnikov Olga Guseva Sergey Bazanovich Sergey Kozlov Alexander Orekhov 

机构地区：[1]Department of problems of atherosclerosis,Chazov National Medical Research Center of Cardiology of the Ministry of Health of the Russian Federation,121552 Moscow,Russia [2]Laboratory of medical genetics,Chazov National Medical Research Center of Cardiology of the Ministry of Health of the Russian Federation,121552 Moscow,Russia [3]Laboratory of cellular and molecular pathology of cardiovascular system,State Scientific Center of the Russian Federation Petrovsky National Research Center of Surgery,Moscow 119991,Russia [4]Laboratory of cell hemostasis,Chazov National Medical Research Center of Cardiology of the Ministry of Health of the Russian Federation,121552 Moscow,Russia [5]Laboratory of Gas Exchange,Biomechanics and Barophysiology,State Scientific Center of the Russian Federation,The Institute of Biomedical Problems of the Russian Academy of Sciences,Moscow 123007,Russia [6]Laboratory of stem cells,Chazov National Medical Research Center of Cardiology of the Ministry of Health of the Russian Federation,121552 Moscow,Russia

出　　处：《Biophysics Reports》2025年第1期12-17,共6页生物物理学报(英文版)

基　　金：supported by Russian Science Foundation(22-15-00134)。

摘　　要：The purpose of this study was to examine the secretion of inflammatory cytokines by cultured monocytes/macrophages in patients with premature coronary artery disease(CAD).The study included 38patients with premature CAD and 35 patients without CAD.A primary culture of CD14+monocytes was obtained by immunomagnetic separation.The inflammatory response was induced by incubation of a cell culture with lipopolysaccharide(LPS)for 24 hours on Days 1 and 6.Basal and LPS-stimulated secretion of the cytokines,tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-8(IL-8)and monocyte chemotactic protein-1(MCP-1)was assessed by enzyme immunoassay on Days 2 and 7 of cultivation.The level of basal secretion of TNF-α,IL-1β,IL-6,MCP-1 was higher in patients with CAD compared to patients in the control group.The levels of re-stimulated TNF-αsecretion and the levels of LPS-stimulated and re-stimulated IL-1βsecretion on the second and sixth days were also higher in patients with CAD.LPS-stimulated MCP-1 secretion on the second day did not differ in patients of both groups,but re-stimulated MCP-1 secretion was higher in patients with CAD.The results of logistic regression analysis showed that the basal secretion levels of IL-1βand IL-6 were independently associated with premature CAD,along with smoking,body mass index and serum HDLcholesterol levels.

关 键 词：Coronary artery disease MONOCYTES Inflammatory cytokines Immune tolerance 

分 类 号：R541.4[医药卫生—心血管疾病]