非小细胞肺癌组织中NDUFA4L2的表达及其临床意义  

Expression of NDUFA4L2 in non-small cell lung cancer and its potential clinical significance

作　　者：韩鹏 张伯翔[3] 杨艺员 李慧婕 许惠 梁一倩 HAN Peng;ZHANG Boxiang;YANG Yiyuan;LI Huijie;XU Hui;LIANG Yiqian(Department of Nuclear Medicine,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China;Department of Otolaryngology-Head and Neck Surgery,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China;Department of Thoracic Surgery,The First Affiliated Hospital of Xi’an Jiaotong University,Xi’an 710061,China)

机构地区：[1]西安交通大学第一附属医院核医学科,陕西西安710061 [2]西安交通大学第一附属医院耳鼻咽喉头颈外科,陕西西安710061 [3]西安交通大学第一附属医院胸外科,陕西西安710061

出　　处：《陕西医学杂志》2025年第4期435-440,457,共7页Shaanxi Medical Journal

基　　金：国家自然科学基金资助青年项目(81902322);陕西省自然科学基础研究计划(2020JQ-526、2023-JC-YB-791);西安交通大学第一附属医院基金资助项目(2021ZYTS-02)。

摘　　要：目的:研究非小细胞肺癌(NSCLC)患者肿瘤及正常肺组织中NDUFA4L2的表达差异,探讨其与患者预后及临床病理特征的关联,以期为NSCLC的诊断与治疗提供新的参考方向。方法:应用Oncomine和癌症基因图谱(TCGA)数据库,比较分析NDUFA4L2在NSCLC与正常肺组织中的表达水平;应用Kaplan-Meier生存曲线分析和Log-rank检验,评估NDUFA4L2表达水平与预后的关系。收集45例NSCLC肿瘤组织标本和癌旁非肿瘤组织,应用RT-PCR及免疫组化方法检测NDUFA4L2的表达情况。结果:通过Oncomine和TCGA数据库分析表明,与正常肺组织比较,肺鳞癌组织NDUFA4L2 mRNA表达量显著增高(P<0.001),而肺腺癌和大细胞肺癌组织中NDUFA4L2 mRNA表达水平与正常肺组织比较无统计学差异(P值分别为0.924和0.165);Kaplan-Meier生存分析指出NDUFA4L2表达水平与肺鳞癌患者总生存期显著相关(P=0.019),且高表达提示预后不良。此外,RT-PCR和免疫组化检测也指出,肺鳞癌组织NDUFA4L2 mRNA和蛋白表达水平较癌旁非肿瘤组织显著增高(P值分别为<0.001和0.008),并且与肺鳞癌的TNM分期呈正相关(P=0.030);肺腺癌组织NDUFA4L2 mRNA和蛋白表达与癌旁非肿瘤组织比较无统计学差异(P值分别为0.567和0.405)。结论:NDUFA4L2在肺鳞癌组织中呈特异性表达,其高表达水平与患者的不良预后密切相关。Objective:To investigate NDUFA4L2 expression in tumor and normal lung tissues of non-small cell lung cancer(NSCLC)patients and analyze its correlation with prognosis and clinicopathological features,providing new insights for the diagnosis and treatment of NSCLC.Methods:The Oncomine and The Cancer Genome Atlas(TCGA)databases were used to compare NDUFA4L2 expression levels in NSCLC and normal lung tissues.The correlation between NDUFA4L2 expression and patient prognosis was analyzed by plotting Kaplan-Meier survival curves and performing log-rank tests.Forty-five NSCLC tumor tissue samples and adjacent nontumor tissues were collected,and NDUFA4L2 expression was detected using RT-PCR and immunohistochemistry(IHC)methods.Results:Analysis of the Oncomine and TCGA databases showed significantly higher mRNA levels of NDUFA4L2 in lung squamous cell carcinoma(LUSC)tissues compared to normal lung tissues(P<0.001).However,no significant differences were observed in NDUFA4L2 mRNA expression between normal lung tissues and those with lung adenocarcinoma(LUAD)or large cell lung carcinoma(P=0.924 and P=0.165,respectively).Kaplan-Meier survival analysis indicated that NDUFA4L2 expression levels were significantly associated with overall survival in patients with LUSC(P=0.019),with high expression indicating a poor prognosis.Additionally,RT-PCR and IHC also showed that the expression levels of NDUFA4L2 mRNA and protein were markedly elevated in LUSC tissues compared to adjacent nontumor tissues(P<0.001 and P=0.008,respectively),and positively correlated with the TNM stage of LUSC(P=0.030).There was no significant difference in NDUFA4L2 mRNA and protein expression between LUAD and adjacent nontumor tissues(P=0.567 and P=0.405,respectively).Conclusion:NDUFA4L2 is specifically expressed in LUSC tissues,and its overexpression is associated with poor prognosis in patients with LUSC.

关 键 词：非小细胞肺癌 肺鳞癌 肺腺癌 NDUFA4L2 预后 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]