高温对厚壳贻贝抗氧化、碳代谢生理指标和转录物组的影响  

Effects of High Temperature on Antioxidant,Carbon Metabolism Physiological Indicators and Transcriptome of Mytilus coruscus

作　　者：杨智 张远悦 王健鑫 廖智 胡群菊 杨巧梅 张晓林 范美华 YANG Zhi;ZHANG Yuan-Yue;WANG Jian-Xin;LIAO Zhi;HU Qun-Ju;YANG Qiao-Mei;ZHANG Xiao-Lin;FAN Mei-Hua(Laboratory of Marine Biology Resource and Molecular Engineering,Marine Science and Technology College,Zhejiang Ocean University,Zhoushan 316022,Zhejiang,China)

机构地区：[1]浙江海洋大学海洋科学与技术学院海洋生物资源与分子工程实验室,浙江舟山316022

出　　处：《中国生物化学与分子生物学报》2025年第3期446-459,共14页Chinese Journal of Biochemistry and Molecular Biology

基　　金：浙江省领雁项目-增强海洋“蓝碳”生态系统汇的关键技术和示范应用(No.2023C03120);国家自然科学基金(No.32271580);国家大学生创新创业项目(No.202310340023);2023年度校级大学生科技创新计划项目(No.2023-A-001)资助。

摘　　要：海洋在全球碳循环中发挥着关键作用,基于“双碳”目标的提出,海洋碳汇受到广泛关注。贝类养殖是形成渔业碳汇的重要来源之一,对近海的碳循环过程产生着重要影响;随着全球温度的升高,海洋酸化现象的加剧,海洋吸收CO_(2)的能力将会发生改变。但是,高温对厚壳贻贝(Mytilus coruscus)碳代谢相关的生理和转录物组的影响不够清晰。本实验主要研究高温对厚壳贻贝总碳含量、碳代谢、抗氧化相关的酶活性以及转录物组的影响。结果表明,高温显著抑制己糖激酶和丙酮酸激酶的活性,而碳酸酐酶的活性增加(P<0.05),降低了消化腺ATP含量(P<0.05),影响了糖酵解和三羧酸循环,导致贻贝固碳能力显著下降。高温导致活性氧、丙二醛、超氧化物歧化酶和过氧化氢酶活性都显著增加(P<0.05)。透射电镜的观察结果显示,高温损伤了厚壳贻贝消化腺的亚细胞结构,使核仁缩小,内质网肿胀,线粒体嵴显著减少。比较转录物组学分析结果显示,高温处理下上调的差异表达基因主要富集在内质网的蛋白质加工(protein processing in endoplasmic reticulum)、抗原的处理和呈递(antigen processing and presentation)以及MAPK信号途径(MAPK signaling pathway)等通路;下调的差异表达基因主要富集于细胞坏死(necroptosis)、DNA复制(DNA replication)和NF-kappa B信号途径(NF kappa B signaling pathway)等通路。抗氧化相关差异基因中,上调差异基因主要有维生素K环氧化物还原酶、过氧化物酶、热休克105 kD蛋白、热休克70 kD蛋白和超氧化物歧化酶等;下调差异表达基因主要包括NADPH氧化酶、谷胱甘肽还原酶、细胞色素b-245、细胞色素P450和醌氧化还原酶等。富集于碳代谢通路的上调基因主要包括转几丁质酶、磷脂酰肌醇4,5-二磷酸3-激酶、磷酸烯醇式丙酮酸羧激酶、半乳糖激酶和三磷酸肌醇3-激酶等;下调基因主要包括醛糖-1-差向异构酶�The ocean plays a critical role in the global carbon cycle,and base on the"dual carbon"goals,ocean carbon sinks have received widespread attention.Shellfish aquaculture is one of the most important sources of carbon sinks in fisheries,which has an important impact on the offshore carbon cycle.As global temperature rises and ocean acidification intensifies,the capacity of the ocean to absorb CO_(2) will change.However,the effects of high temperature on the physiology and transcriptome related to carbon metabolism in Mytilus coruscus are not clear enough.This study investigated the effects of high temperatures on the total carbon content,carbon metabolism,antioxidant-related enzyme activities,and the transcriptome of Mytilus coruscus.The results showed that high temperature significantly inhibited the activities of hexokinase and pyruvate kinase,increased carbonic anhydrase activity(P<0.05),decreased the ATP content of digestive glands(P<0.05),and affected glycolysis and the tricarboxylic acid cycle,leading to a significant decrease in the mussel’s ability to sequester carbon.High temperature resulted in significant(P<0.05)increases in the levels of reactive oxygen species and malondialdehyde,and enhanced the activities of superoxide dismutase and catalase.Observations by transmission electron microscopy showed that high temperatures damaged the subcellular structure of the digestive gland in Mytilus coruscus,resulting in the shrinkage of the nucleolus,swelling of the endoplasmic reticulum,and a significant reduction in the mitochondrial cristae.Comparative transcriptomic analysis showed that the upregulated DEGs were mainly enriched in protein processing in the endoplasmic reticulum,antigen processing and presentation,and MAPK signaling pathway.The downregulated DEGs were mainly enriched in necroptosis,DNA replication,and the NF-kappa B signaling pathway.In antioxidant-related DEGs,the upregulated DEGs include vitamin K epoxide reductase,peroxidases,heat shock protein 105 kD,heat shock protein 70 kD,and superoxi

关 键 词：高温 厚壳贻贝 固碳 生理 转录物组 

分 类 号：Q789[生物学—分子生物学]