High-fat intake induces gut microbiota disorders,inflammatory responses and oxidative stress in Nyctereutes procyonoides  

作　　者：Zhaoming Li Chengwei Wei Jie Yang Yuan Geng Mengran Zhu Tianchao Xu Mengyao Guo 

机构地区：[1]College of Veterinary Medicine,Northeast Agricultural University,Har-bin 150030,China [2]Heilongjiang Dongbeinongda Animal Hospital Ltd.,Harbin 150038,China

出　　处：《Animal Diseases》2025年第1期17-32,共16页动物疾病(英文)

摘　　要：The Nyctereutes procyonoides is highly regarded in the farming and leather industries because of the high value of its fur,which renders artificial feeding a crucial aspect.However,high-fat diets have always been associated with a variety of digestive disorders.This study aimed to investigate the impact of high-fat diets on the gut microbiota and the mechanisms of gut damage in Nyctereutes procyonoides.16S rRNA sequencing demonstrated that high-fat diets caused diarrhea and intestinal damage through alterations in the gut microbiota:a decrease in the abundance of Firmicutes,an increase in the abundance of Proteobacteria and Actinobacteria,and an increase in the abundance of Enterococcaceae,Escherichia coli-Shigella,Clostridium and Lactobacillus.Subsequently,changes in metabolic path-ways,such as amino and fatty acid pathways,were identified by KEGG and COG enrichment analysis,and the TLR4/NF-κB/NLRP3 inflammatory signaling pathway was shown to be activated by high-fat diets.In addition,high-fat diets lead to the accumulation of ROS and MDA and reduce the activity of the antioxidant enzymes GSH-PX and SOD.C orrespondingly,the levels of proinflammatory cytokines(IL-6,IL-1βand TNF-α)were significantly increased,and the apoptosis and necrosis signaling pathways of colonic cells were detected,causing a dramatic decrease in the expression of intestinal tight junction proteins(Occludin,E-cadherin,ZO-1 and ZO-2).In conclusion,high-fat diets altered the structure of the Nyctereutes procyonoides gut microbiota community and led to colon damage.This study provides new insights into the intestinal health of Nyctereutes procyonoides.

关 键 词：Nyctereutes procyonoides High-fat diets Gut microbiota Inflammatory responses Oxidative stress Intestinal barrier 

分 类 号：R57[医药卫生—消化系统]