miR-223-3p通过靶向NLRP3恢复高血压脑出血大鼠的认知功能  

miR-223-3p recover cognitive function in rats with hypertensive intracerebral hemorrhage by targeting NLRP3

作　　者：余梦擎 吴金波 赖志勇 陈忠强 YU Mengqing;WU Jinbo;LAI Zhiyong;CHEN Zhongqiang(Emergency Medicine Center,the Affiliated Hospital of Guangdong Medical University,Zhanjiang 524001,China)

机构地区：[1]广东医科大学附属医院急诊医学中心,广东湛江524001

出　　处：《新医学》2025年第3期268-274,共7页Journal of New Medicine

基　　金：广东省医学科研基金-青年项目(A2023578)。

摘　　要：目的 探索miR-223-3p对高血压脑出血大鼠认知功能恢复的影响及其调控机制。方法 构建高血压脑出血大鼠模型,分析miR-223-3p表达和认知功能改变;过表达/敲降miR-223-3p,探讨其对NOD样受体家族含pyrin结构域3(NLRP3)炎症小体表达和大鼠认知功能的影响;敲降NLRP3,确认该炎症小体对脑出血大鼠认知功能的作用;通过荧光素酶实验鉴定miR-223-3p对NLRP3的直接靶向作用。结果 高血压脑出血大鼠的脑组织中miR-223-3p表达上调。miR-223-3p抑制NLRP3的表达,并恢复大鼠认知功能。荧光素酶实验结果表明miR-223-3p可以直接靶向NLRP3的mRNA并下调其表达。结论 miR-223-3p通过与NLRP3的mRNA结合下调NLRP3的表达,从而恢复脑出血大鼠的认知功能。Objective To explore the effects of miR-223-3p on cognitive function recovery in rats with hypertensive intracerebral hemorrhage(HICH)and its regulatory mechanisms.Methods The rat model of HICH was constructed to analyze changes in miR-223-3p expression and cognitive function.Subsequently,miR-223-3p was overexpressed or knocked down to investigate its effects on the expression of the inflammasome NLRP3 and cognitive function in rats.NLRP3 was then knocked down to confirm its role in cognitive function in HICH rats.Finally,a luciferase assay was performed to identify the direct targeting effect of miR-223-3p on NLRP3.Results The expression of miR-223-3p was upregulated in the brain tissues of HICH rats.miR-223-3p inhibits NLRP3 expression and recovers cognitive function in rats.The luciferase assay demonstrated that miR-223-3p could directly target the mRNA of NLRP3 and downregulate its expression.Conclusion miR-223-3p downregulates NLRP3 expression by binding to its mRNA,thereby promoting the recovery of cognitive function in rats with intracerebral hemorrhage.

关 键 词：miR-223-3p 炎症小体 NLRP3 高血压 脑出血 大鼠 

分 类 号：R743.34[医药卫生—神经病学与精神病学]