Bioactivity of dressings based on platelet-rich plasma and Plateletrich fibrin for tissue regeneration in animal model  

作　　者：João Abel Sá-Oliveira Monique Vieira Geraldo Milena Marques Rafael Messias Luiz Filipe Krasinski Cestari Ingrid Nascimento Lima ThaynáCristine De Souza Ana Carla Zarpelon-Schutz Kádima Nayara Teixeira 

机构地区：[1]Programa Multicêntrico de Pós-graduação em Bioquímica e Biologia Molecular-Setor Palotina,Universidade Federal do Paraná,Palotina 85950-000,Paraná,Brazil [2]Campus Toledo,Universidade Federal do Paraná,Toledo 85919-899,Paraná,Brazil [3]Patologia,Laboratório Prevenção Patologia,Cascavel 85810-040,Paraná,Brazil [4]Medicina-Campus Toledo,UFPR-Universidade Federal do Paraná,Toledo 85919-899,Paraná,Brazil [5]Programa de Pós-graduação em Biotecnologia-Setor Palotina,Universidade Federal do Paraná,Palotina 85950-000,Paraná,Brazil

出　　处：《World Journal of Biological Chemistry》2025年第1期10-19,共10页世界生物化学杂志(英文)

摘　　要：BACKGROUND Skin wounds are common injuries that affect quality of life and incur high costs.A considerable portion of healthcare resources in Western countries is allocated to wound treatment,mainly using mechanical,biological,or artificial dressings.Biological and artificial dressings,such as hydrogels,are preferred for their biocompatibility.Platelet concentrates,such as platelet-rich plasma(PRP)and platelet-rich fibrin(PRF),stand out for accelerating tissue repair and minimizing risks of allergies and rejection.This study developed PRF and PRP-based dressings to treat skin wounds in an animal model,evaluating their functionality and efficiency in accelerating the tissue repair process.AIM To develop wound dressings based on platelet concentrates and evaluating their efficiency in treating skin wounds in Wistar rats.METHODS Wistar rats,both male and female,were subjected to the creation of a skin wound,distributed into groups(n=64/group),and treated with Carbopol(negative control);PRP+Carbopol;PRF+Carbopol;or PRF+CaCl_(2)+Carbopol,on days zero(D0),D3,D7,D14,and D21.PRP and PRF were obtained only from male rats.On D3,D7,D14,and D21,the wounds were analyzed for area,contraction rate,and histopathology of the tissue repair process.RESULTS The PRF-based dressing was more effective in accelerating wound closure early in the tissue repair process(up to D7),while PRF+CaCl_(2) seemed to delay the process,as wound closure was not complete by D21.Regarding macroscopic parameters,animals treated with PRF+CaCl_(2) showed significantly more crusting(necrosis)early in the repair process(D3).In terms of histopathological parameters,the PRF group exhibited significant collagenization at the later stages of the repair process(D14 and D21).By D21,fibroblast proliferation and inflammatory infiltration were higher in the PRP group.Animals treated with PRF+CaCl_(2) experienced a more pronounced inflammatory response up to D7,which diminished from D14 onwards.CONCLUSION The PRF-based dressing was effective in accelerating the closur

关 键 词：Skin wound Murine model Platelet-rich fibrin Platelet-rich plasma Tissue repair 

分 类 号：R78[医药卫生—口腔医学]