过氧化物还原酶-4在口腔鳞状细胞癌中的表达及对癌细胞增殖、迁移和侵袭的影响  

Expression of peroxiredoxin 4 in oral squamous cell carcinoma and its effects on cancer cell proliferation,mi⁃gration,and invasion

作　　者：耿华 李磊 杨杰 柳云霞 陈晓东 GENG Hua;LI Lei;YANG Jie;LIU Yunxia;CHEN Xiaodong(Department of Stomatology,Weifang Hospital of Traditional Chinese Medicine,Weifang 261000,China;Shouguang Stomatological Hospital,Weifang 262700,China;Department of Stomatology,Affiliated Hospital of Shandong Second Medical University,Weifang 266000,China;School of Stomatology,Shandong Second Medical University,Weifang 261053,China;Department of Stomatology,Zhucheng People's Hospital Affiliated to Shandong Second Medical University,Zhucheng 262200,China)

机构地区：[1]潍坊市中医院口腔科,山东潍坊261000 [2]山东省寿光市口腔医院,山东潍坊262700 [3]山东第二医科大学附属医院口腔科,山东潍坊266000 [4]山东第二医科大学口腔医学院,山东潍坊261053 [5]山东第二医科大学附属诸城市人民医院口腔科,山东诸城262200

出　　处：《口腔疾病防治》2025年第4期278-288,共11页Journal of Prevention and Treatment for Stomatological Diseases

基　　金：山东省自然科学基金面上项目(ZR2020MH188)。

摘　　要：目的 探讨过氧化物还原酶-4(peroxiredoxin-4,PRDX4)在口腔鳞状细胞癌(oral squamous cell carcinoma,OSCC)中的表达及对OSCC细胞增殖、迁移和侵袭的影响。方法 通过癌症基因图谱数据库(The Cancer GenomeAtlas,TCGA)分析PRDX4在OSCC中的表达,实时荧光定量聚合酶链式反应(real-time fluorescent quantitative polymerase chain reaction,qRT-PCR)和蛋白质印迹实验(Western Blot,WB)分别检测OSCC细胞系中PRDX4的基因与蛋白质表达。将CAL-27细胞中的PRDX4敲低,分为si-PRDX4组和si-NC组;将SCC-9细胞中的PRDX4过表达,分为过表达PRDX4组(转染pcDNA3.1-PRDX4质粒)和Vector组(对照组,转染pcDNA3.1-NC质粒)。采用细胞计数试剂盒(cell counting kit-8,CCK-8)和平板克隆形成实验检测各组细胞增殖能力;细胞划痕和Transwell侵袭实验检测细胞迁移和侵袭能力;WB实验检测敲低和过表达PRDX4及加入p38MAPK激动剂和抑制剂后对OSCC细胞中与p38MAPK相关信号通路蛋白及上皮间充质转化(epithelial-mesenchymal transition,EMT)蛋白表达影响。结果 PRDX4在OSCC组织及细胞系中呈高表达。CCK-8实验结果显示,si-PRDX4组较si-NC组在24、48和72 h时OD值低(P<0.05);过表达PRDX4组较Verctor组在24、48和72 h时OD值高(P<0.05)。平板克隆形成实验结果显示,si-PRDX4组较si-NC组集落形成数量少(P<0.05);过表达PRDX4组较Vector组集落形成数量多(P<0.05)。细胞划痕实验结果显示,si-PRDX4组较si-NC组划痕愈合面积少(P<0.05);过表达PRDX4组较Vector组划痕愈合面积增多(P<0.05)。Transwell侵袭实验结果显示,siPRDX4组较si-NC组穿膜细胞数量减少(P<0.05);过表达PRDX4组较Verctor组穿膜细胞数量多(P<0.05)。WB实验结果显示,敲低和过表达PRDX4可分别下调和上调p38MAPK信号通路及上皮间充质转化相关蛋白表达,而分别加入p38MAPK激动剂和抑制剂后,可显著逆转相关蛋白的表达。结论 PRDX4在OSCC中处于高表达状态,敲低OSCC细胞中PRDX4的表达,可下调p38 MObjective To investigate the expression of peroxiredoxin 4(PRDX4)in oral squamous cell carcinoma(OSCC)and its effect on the proliferation,migration,and invasion of OSCC cells.Methods The Cancer Genome Atlas(TCGA)database was used to analyze the expression of PRDX4 in OSCC.Quantitative reverse transcription polymerase chain reaction(qRT-PCR)and Western Blot(WB)were used to detect the mRNA and protein expression of PRDX4 in OSCC cell lines and normal oral mucosal epithelial cells.PRDX4 was knocked down in CAL-27 cells and divided into two groups:the si-PRDX4 group and si-NC group.SCC-9 cells overexpressing PRDX4 were divided into two groups:the PRDX4 overexpression group(transfected with pcDNA3.1-PRDX4 plasmid)and the vector group(the control group;transfected with pcDNA3.1-NC plasmid).A cell counting kit-8(CCK-8)and plate colony formation assay were used to detect cell proliferation.Transwell assay and cell scratch test were used to detect cell invasion and migration ability.WB was used to detect the effects of knockdown or overexpression of PRDX4,p38MAPK agonist or inhibitor on the expression of p38MAPK-related signaling pathway proteins,and epithelial mesenchymal transition proteins in OSCC cells.Results PRDX4 was highly expressed in OSCC tissues and cell lines.The results of qRT-PCR and WB showed that PRDX4 was highly expressed in OSCC cell lines compared with normal oral mucosal epithelial cells.The CCK-8 assay showed that the si-PRDX4 group had significantly lower OD values than the si-NC group at 24,48,and 72 h(P<0.05).The PRDX4 overexpression group had a significantly higher OD value than the vector group at 24,48,and 72 h(P<0.05).The plate colony formation assay showed that the si-PRDX4 group had a significantly lower number of colonies than the si-NC group(P<0.05).The number of colonies formed in the PRDX4 overexpression group was significantly higher than that in the vector group(P<0.05).The cell scratch test showed that the wound healing area of the si-PRDX4 group was less than that of the si-NC group(P

关 键 词：氧化物还原酶-4 促癌因子 口腔鳞状细胞癌 P38MAPK信号通路 上皮间充质转化 增殖 侵袭 迁移 

分 类 号：R78[医药卫生—口腔医学]