晚期胃癌患者外周血单个核细胞中AIM2的表达及其与肠道菌群和靶向治疗疗效的关系研究  

Study on the Expression of AIM2 in PBMC of Patients with Advanced Gastric Cancer and Its Relationship with Intestinal Microbiota and the Efficacy of Targeted Therapy

作　　者：李爱丽 魏宏英 高先凤 LI Aili;WEI Hongying;GAO Xianfeng(Department of Oncology,Sinopharm Tongmei General Hospital,Shanxi Datong 037003,China)

机构地区：[1]国药同煤总医院肿瘤内科,山西大同037003

出　　处：《现代检验医学杂志》2025年第2期6-10,共5页Journal of Modern Laboratory Medicine

基　　金：山西省基础研究计划青年科学研究项目,编号:202203021222351。

摘　　要：目的探讨晚期胃癌(GC)患者外周血单个核细胞(PBMC)中黑素瘤缺乏因子2(AIM2)基因表达及其与肠道菌群和靶向治疗疗效的关系。方法选取2023年1~12月收治的83例晚期GC患者,均给予曲妥珠单抗/纳武利尤单抗联合常规化疗治疗,4周/疗程,持续治疗3个疗程,评价靶向治疗疗效。利用16S rDNA测序技术检测患者肠道菌群丰度;实时荧光定量PCR(qRT-PCR)检测PBMC中AIM2mRNA表达水平,按其中位数分为高表达组和低表达组;采用Pearson相关性分析AIM2表达与肠道菌群相对丰度、靶向治疗疗效及临床特征的关系。结果在门水平上,AIM2高表达组拟杆菌门(0.70±0.15)和变形菌门(0.71±0.21)相对丰度明显低于低表达组(0.81±0.17,0.80±0.16),厚壁菌门(0.73±0.12)相对丰度明显高于低表达组(0.64±0.08),差异具有统计学意义(t=3.108,2.210,4.061,均P<0.05);在属水平上,AIM2高表达组拟杆菌属(0.13±0.02)相对丰度明显低于低表达组(0.19±0.04),肠球菌属(0.31±0.08)和大肠埃希菌属(0.18±0.04)相对丰度明显高于低表达组(0.12±0.05,0.10±0.02),差异具有统计学意义(t=8.472,15.462,11.722,均P<0.05)。Pearson相关分析显示,门水平上,AIM2表达与厚壁菌门相对丰度呈正相关(r=0.598,P<0.05),与拟杆菌门和变形菌门相对丰度呈负相关(r=-0.641,-0.520,均P<0.05);属水平上,AIM2表达与肠球菌属和大肠埃希菌属相对丰度呈正相关(r=0.529,0.577,均P<0.05),与拟杆菌属相对丰度呈负相关(r=-0.574,P<0.05)。83例晚期GC患者中靶向治疗有效17例,靶向治疗无效66例,总客观缓解率(ORR)为20.48%;AIM2高表达组病灶直径(5.73±0.74cm)和cTNM分期IV期占比(53.85%)显著低于低表达组(6.08±0.51cm,75.00%),靶向治疗ORR(30.77%)高于低表达组(11.36%),差异具有统计学意义(t/χ^(2)/Z=2.477,6.558,4.780,均P<0.05)。结论晚期GC患者PBMC中AIM2表达与患者肠道菌群失调及靶向治疗效果密切相关,检测AIM2表达有利于指导临床筛选出对靶向�Objective To investigate the relationship between the expression of absent in melanoma 2(AIM2)gene in peripheral blood peripheral blood mononuclear cell(PBMC)of patients with advanced gastric cancer(GC)and the intestinal flora and the efficacy of targeted therapy.Methods 83 patients with advanced GC who were treated from January to December 2023 were all given Trastuzumab/Nebuliumab combined with conventional chemotherapy for 4 weeks/course and 3 continuous courses of treatment to evaluate the efficacy of targeted therapy.The abundance of intestinal flora was analyzed using 16S rDNA sequencing technologies.Quantitative real-time PCR(qRT-PCR)was used to detect AIM2 mRNA expression levels in PBMC,which were divided into a high expression group and a low expression group according to the median digit.Pearson correlation analysis was used to investigate the relationship between AIM2 expression and the relative abundance of intestinal flora,the efficacy of targeted therapy and clinical features.Results At the phylum level,the relative abundance of Bacteroidetes(0.70±0.15)and Proteobacteria(0.71±0.21)in the high expression group of AIM2 was significantly lower than that in the low expression group(0.81±0.17,0.80±0.16),and the relative abundance of Firmicutes(0.73±0.12)was significantly higher than that in the low expression group(0.64±0.08),and the differences were statistically significant(t=3.108,2.210,4.061,all P<0.05).At the genus level,the relative abundance of Bacteroides(0.13±0.02)in the high expression group of AIM2 was significantly lower than that in the low expression group(0.19±0.04),and the relative abundance of Enterococcus(0.31±0.08 vs 0.12±0.05)and Escherichia coli(0.18±0.04 vs 0.10±0.02)was significantly higher than that in the low expression group(0.12±0.05,0.10±0.02),and the differences were statistically significant(t=8.472,153.462,11.722,all P<0.05).Pearson correlation showed that at phylum level,AIM2 expression was positively correlated with the relative abundance of Firmicutes(r=0.5

关 键 词：晚期胃癌 黑素瘤缺乏因子2 肠道菌群 靶向治疗 

分 类 号：R735.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]