非小细胞肺癌组织中ADH5 mRNA,FUNDC1 mRNA的表达与临床病理特征及预后的关系研究  

Relationship between Expression of ADH5 mRNA,FUNDC1 mRNA and Clinicopathological Features and Prognosis in Non-Small Cell Lung Cancer

作　　者：杜亮[1] 阿布都买拉木·阿布都吾甫尔 金澄宇[1] 马金山[1] DU Liang;ABUDUMAIRAMU Abudumufur;JIN Chengyu;MA Jinshan(Department of Thoracic Surgery,People’s Hospital of Xinjiang Uygur Autonomous Region,Urumqi 830000,China)

机构地区：[1]新疆维吾尔自治区人民医院胸外科,乌鲁木齐830000

出　　处：《现代检验医学杂志》2025年第2期36-40,共5页Journal of Modern Laboratory Medicine

基　　金：新疆维吾尔自治区自然科学基金(2020D01A112);新疆维吾尔自治区人民医院项目(20180317)。

摘　　要：目的研究非小细胞肺癌(NSCLC)组织中乙醇脱氢酶5(ADH5)mRNA,FUN14结构域蛋白1(FUNDC1)mRNA的表达与临床病理特征及预后的关系。方法收集2019年7月~2021年7月在新疆维吾尔自治区人民医院接受治疗并经术后病理确诊为NSCLC的332例患者的临床资料。应用实时荧光定量PCR(qRT-PCR)法测定NSCLC组织及相应的癌旁组织(距癌组织边缘>5cm)中ADH5 mRNA和FUNDC1 mRNA的表达情况,并通过t检验分析其表达差异及其与NSCLC患者临床病理特征的相关性;采用Pearson等级相关分析ADH5 mRNA与FUNDC1 mRNA的相关性;Kaplan-Meier法分析ADH5 mRNA和FUNDC1 mRNA表达与NSCLC患者预后的关系。结果qRT-PCR结果显示,NSCLC组织中ADH5 mRNA表达水平(0.56±0.30)显著低于癌旁正常组织(1.03±0.50),而FUNDC1 mRNA表达水平(2.91±1.02)显著高于癌旁正常组织(1.20±0.83),差异具有统计学意义(t=14.687,23.694,均P<0.05);且Pearson法相关检验显示,ADH5 mRNA与FUNDC1 mRNA在NSCLC组织的表达呈显著负相关(r=-0.869,P=0.006)。年龄<55岁、TNM分期III期、低分化、并发淋巴结转移的NSCLC组织中ADH5 mRNA表达低于年龄≥55岁、TNM分期Ⅰ/Ⅱ期、高/中分化、无淋巴结转移(t=1.866~6.087);低分化、并发淋巴结转移的NSCLC组织中FUNDC1 mRNA表达高于高/中分化、无淋巴结转移(t=4.652,2.787),差异具有统计学意义(均P<0.05)。Kaplan-Meier生存分析显示,ADH5高表达组的累积总生存率(66.99%)显著高于ADH5低表达组(54.15%),而FUNDC1低表达组累积总生存率(69.47%)显著高于FUNDC1高表达组(53.59%),差异具有统计学意义(Log Rank χ^(2)=5.760,5.165,P=0.016,0.023)。结论在NSCLC的患者肿瘤组织中ADH5 mRNA呈低表达,FUNDC1 mRNA呈高表达,两者可能共同参与NSCLC发生及转移,有望成为诊疗及预后预测的参考指标。Objective To investigate the expression of alcohol dehydrogenase 5(ADH5)mRNA and FUN14 domain containing 1(FUNDC1)mRNA in non-small cell lung cancer(NSCLC)tissues and their relationship with clinical pathological characteristics and prognosis.Methods Collected clinical data of 332 patients who received treatment and non-small cell lung cancer(NSCLC)diagnosed through postoperative puthology at Xinjiang Uygur Autonomous Region People’s Hospital from July 2019 to July 2021.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to measure the expression of ADH5 mRNA and FUNDC1 mRNA in NSCLC tissues and corresponding adjacent tissues(>5cm from the edge of the cancer tissue),and the expression differences and their correlation with the clinical and pathological characteristics of NSCLC patients were analyzed by t-test.Pearson rank correlation analysis was used to investigate the correlation between ADH5 mRNA and FUNDC1 mRNA.Kaplan-Meier method was used to analyze the relationship between the expression of ADH5 mRNA and FUNDC1 mRNA and the prognosis of NSCLC patients.Results The results of qRT-PCR showed that the expression level of ADH5 mRNA(0.56±0.30)in NSCLC tissues was significantly lower than that in paracancer normal tissues(1.03±0.50),and the expression level of FUNDC1 mRNA(2.91±1.02)was significantly higher than that in paracancer normal tissues(1.20±0.83),and the differences were statistically significant(t=14.687,23.694,all P<0.05).Correlation test by Pearson method showed that the expression of ADH5 mRNA and FUNDC1 mRNA in NSCLC tissues was significantly negatively correlated(r=-0.869,P=0.006).The expression of ADH5 mRNA in NSCLC tissues with age<55 years,TNM stage III,low differentiation and concurrent lymph node metastasis was lower than that in NSCLC tissues with age≥55 years,TNM stage I/II,high/moderate differentiation and no lymph node metastasis(t=1.866~6.087),the expression of FUNDC1 mRNA in NSCLC tissues with low differentiation and concurrent lymph node metastasis was higher than t

关 键 词：非小细胞肺癌 乙醇脱氢酶5 FUN14结构域蛋白1 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]