TIMP-2基因418 C>G位点多态性与非小细胞肺癌易感性的相关性研究  

Study on the Correlation between Polymorphism of TIMP-2 Gene 418 C>G Locus and Susceptibility to NSCLC

作　　者：刘雪梅 沈娜 LIU Xuemei;SHEN Na(Department of Oncology,Suining Central Hospital,Sichuan Suining 629000,China)

机构地区：[1]遂宁市中心医院肿瘤内科,四川遂宁629000

出　　处：《现代检验医学杂志》2025年第2期41-46,共6页Journal of Modern Laboratory Medicine

摘　　要：目的探讨组织金属蛋白酶抑制剂-2(TIMP-2)基因418 C>G位点多态性与非小细胞肺癌(NSCLC)易感性的关系。方法选择2021年5月~2023年5月于遂宁市中心医院收治的167例NSCLC患者作为观察组,选择同期于该院进行体检的183例健康者作为对照组。比较两组的一般资料及分析TIMP-2基因多态性。检测并比较两组TIMP-2基因多态性位点基因型及等位基因频率分布。再通过Logistic回归分析TIMP-2基因418 C>G位点与NSCLC的相关性。结果观察组的BMI≥24kg/m^(2)占比(67.07%)和吸烟人数占比(61.08%)高于对照组(44.26%和40.44%),差异具有统计学意义(χ^(2)=18.356,14.881,均P<0.001)。变异型GG纯合子产生的3个长度为230bp,51bp和23bp的片段,野生型CC纯合子产生长度为253bp和51bp的片段,杂合子GC产生的4个长度为253bp,230bp,51bp和23bp的片段,其中51bp和23bp片段不显示,TIMP-2418 C>G基因一致性为100%。基因分型频率满足Hardy-Weinberg遗传平衡(P>0.05)。观察组TIMP-2基因型CC,GC,GG基因频率分别为10.18%,41.92%和47.90%,对照组分别为4.37%,37.70%和57.92%,差异具有统计学意义(χ^(2)=6.163,P=0.046)。观察组TIMP-2等位基因C,G频率分别为31.14%,68.86%,对照组分别为23.22%,76.78%,差异具有统计学意义(χ^(2)=5.549,P=0.018)。与对照组比较,Logistic回归分析发现携带C等位基因明显增加人群患NSCLC的发病风险(OR=2.669,95%CI:1.478~2.936,P<0.01);与GG+GC型组相比,CC型基因患者的染色体核型预后不良占比、国际预后积分系统(IPSS)危险分组中高危占比、C反应蛋白(CRP)以及降钙素原(PCT)水平升高,差异具有统计学意义(t/χ^(2)=3.633~11.249,均P<0.001);中性粒细胞绝对计数(ANC)、血红蛋白(HGB)、血小板(PLT)水平降低,差异具有统计学意义(t=4.760,2.015,2.212,均P<0.05)。多因素COX回归分析结果显示,CC型基因、染色体核型、IPSS危险分组、ANC,HGB,PLT,CRP,PCT均为NSCLC患者预后不良的危险因素(Wald χ^(2)=3.072~17.611,均Objective To investigate the relationship between 418 C>G polymorphism of tissue metalloproteinase inhibitor-2(TIMP-2)gene and susceptibility to non-small cell lung cancer(NSCLC).Methods A total of 167 patients with NSCLC admitted to Suining Central Hospital from May 2021 to May 2023 were selected as the observation group,and 183 healthy subjects who underwent physical examination in the hospital during the same period were selected as the control group.The general data of the two groups were compared and the polymorphism of the TIMP-2 gene was analyzed.The genotype and allele frequency distribution of the polymorphic locus of the TIMP-2 gene were detected and compared between the two groups.Logistic regression was used to analyze the correlation between TIMP-2 gene 418 C>G site and NSCLC.Results The proportion of BMI≥24kg/m^(2) and smoking in the observation group were 67.07% and 61.08%,respectively,which were 44.26% and 40.44%in the control group,and the differences were statistically significant(χ^(2)=18.356,14.881,all P<0.001).The variant GG homozygote produced three fragments of 230bp,51bp and 23bp in length,the wild-type CC homozygote produced 253bp and 51bp in length,and the heterozygous GC produced four pieces of 253bp,230bp,51bp and 23bp in length.The 51bp and 23bp fragments were not shown,and the TIMP-2418 C>G gene identity was 100%.The genotype frequencies met the Hardy-Weinberg genetic equilibrium(P>0.05).The frequencies of CC,GC and GG of the TIMP-2 genotype in the observation group were 10.18%,41.92% and 47.90%,respectively,and those in the control group were 4.37%,37.70% and 57.92%,respectively,and the difference was statistically significant(χ^(2)=6.163,P=0.046).The frequencies of the C and G alleles of TIMP-2 in the observation group were 31.14% and 68.86%,respectively,and those in the control group were 23.22% and 76.78%,respectively,and the difference was statistically significant(χ^(2)=5.549,P=0.018).Compared with the control group Logistic regression analysis showed that genotype carryin

关 键 词：组织金属蛋白酶抑制剂-2基因 基因多态性 非小细胞肺癌 易感性 

分 类 号：R734.2[医药卫生—肿瘤] R730.43[医药卫生—临床医学]