超声辅助低共熔溶剂提取-高效液相色谱法分析“紫娟”茶中花青素  

Determination of anthocyanins in“Zijuan”tea by ultrasound-assisted deep eutectic solvent extraction combined with high performance liquid chromatography

作　　者：罗发美 李宗师 黄晨 牛之瑞 彭翔宇 金红萍 谭文涵 朱臆霖 LUO Famei;LI Zongshi;HUANG Chen;NIU Zhirui;PENG Xiangyu;JIN Hongping;TAN Wenhan;ZHU Yilin(National Pu’er Tea Product Quality Supervision and Inspection Center,Pu’er 665099,Yunnan,China;College of Pu’er Tea,West Yunnan University of Applied Science,Pu’er 665099,Yunnan,China;Yunnan Institute of Product Quality Supervision&Inspection,Kunming 650223,Yunnan,China;Pu'er Tea Investment(Group)Co.,Ltd,Pu’er 665099,Yunnan,China)

机构地区：[1]国家普洱茶产品质量检验检测中心,云南普洱665099 [2]滇西应用技术大学普洱茶学院,云南普洱665099 [3]云南省产品质量监督检验研究院,云南昆明650223 [4]普洱茶投资(集团)有限公司,云南普洱665099

出　　处：《云南大学学报(自然科学版)》2025年第2期355-364,共10页Journal of Yunnan University(Natural Sciences Edition)

基　　金：云南省乡村振兴科技专项(20231135);“茶城英才支持计划”质量基础高层次创新团队;科技创新平台建设—茶叶咖啡质量基础与应用研究(202407AB110023)。

摘　　要：建立一种高效液相色谱法检测“紫娟”茶中6种花青素的分析方法;样品经由氯化胆碱、草酸(摩尔比为1∶2)制备的低共熔溶剂提取,60℃温度条件下超声提取20 min后,外标法定量分析“紫娟”茶中6种花青素含量;6种花青素在0.1~5 mg/L质量浓度范围内,线性相关系数良好,相关系数均大于0.9994,方法检出限0.2~0.5 mg/kg,定量限0.8~1.7 mg/kg,在0.5、2.5、5.0 mg/kg 3个不同质量浓度添加水平下平均回收率为70.4%~94.3%,相对标准偏差为1.1%~11.3%.建立的超声辅助低共熔溶剂提取-高效液相色谱分析“紫娟”茶中花青素的方法,具有较好的特异性,简单环保,提取效率优于传统方法,为“紫娟”茶中花青素的提取提供了一种新方法,具有良好的发展前景和应用潜力.An analytical method was established for the determination of 6 kinds of anthocyanins in“Zijuan”tea with high performance liquid chromatography(HPLC).The samples were extracted using a deep eutecticsolvent(DES)prepared with choline chloride and oxalic acid(molar ratio 1∶2),after ultrasonic extraction at 60℃for 20 minutes,the content of six anthocyanins in"Zijuan"tea was quantitatively analyzed using an externalstandard method.The calibration curves of the six anthocyanins showed good linearity with correlation coefficientsof above 0.9994 in the range of 0.1-5 mg/L.Limit of the detection(LOD)and the limit of the quantitative(LOQ)were 0.2-0.5 mg/g and 0.8-1.7 mg/g respectively.The recoveries at 0.5,2.5,5.0 mg/kg were 70.4%-94.3%withrelative standard deviations of 1.1%-11.3%.The established method of ultrasound assisted deep eutectic solventextraction and high-performance liquid chromatography for analyzing anthocyanins in“Zijuan”tea has goodspecificity,is simple and environmentally friendly,and has better extraction efficiency than traditional methods.This research provides a new method for extracting anthocyanins from“Zijuan”tea,which has good developmentprospects and application potential.

关 键 词：低共熔溶剂 “紫娟”茶 花青素 高效液相色谱法 

分 类 号：O657[理学—分析化学]