三脉菝葜根茎水部位化学成分及其生物活性研究  

Chemical constituents from the water fraction of rhizoma of Smilax trinervula and their biological activities

作　　者：梁永红 汪嘉诚 黄慧莲 姚慧莹 鲁遇 王诚琪 钟海莹 余英才[3] 张海燕 LIANG Yong-hong;WANG Jia-cheng;HUANG Hui-lian;YAO Hui-ying;LU Yu;WANG Cheng-qi;ZHONG Hai-ying;YU Ying-cai;ZHANG Hai-yan(Key Laboratory of Modem Preparation of Traditional Chinese Medicine,Ministry of Education,Jiangxi University of Chinese Medicine,Nanchang 330004,China;Library,Jiangxi University of Chinese Medicine,Nanchang 330004,China;College of Chinese medicine,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004 [2]江西中医药大学图书馆,江西南昌330004 [3]江西中医药大学中医学院,江西南昌330004

出　　处：《中成药》2025年第3期807-812,共6页Chinese Traditional Patent Medicine

基　　金：国家自然科学基金项目(81560640);江西省自然科学基金项目(20171BAB205089);江西省教育厅科学技术研究项目(GJJ190661);中药改良创新江西省重点实验室(2024SSY07131)。

摘　　要：目的研究三脉菝葜根茎水部位的化学成分及其生物活性。方法采用聚酰胺、硅胶、Sephadex LH-20、ODS及半制备HPLC进行分离纯化,根据理化性质及波谱数据鉴定所得化合物的结构。采用MTT法测定抗肿瘤活性,PNPG法测定α-葡萄糖苷酶抑制活性。结果从中分离得到11个化合物,分别鉴定为酪氨酸(1)、尿嘧啶核苷(2)、2-(2′,3′,4′-三羟基丁基)-6-(2″,3″,4″-三羟基丁基)-吡嗪(3)、2-(1′,2′,3′,4′-四羟基丁基)-6-(2″,3″,4″-三羟基丁基)-吡嗪(4)、2-(1′,2′,3′,4′-四羟基丁基)-5-(2″,3″,4″-三羟基丁基)-吡嗪(5)、尿嘧啶(6)、2-(1′,2′,3′,4′-四羟基丁基)-5-(1″,2″,3″,4″-四羟基丁基)-吡嗪(7)、薯蓣皂苷(8)、莽草酸(9)、吡嗪(10)、3,4-二羟基苯乙醇-8-O-β-D-葡萄糖苷(11)。化合物8对人乳腺癌细胞MCF-7的IC_(50)值为(2.36±0.26)μg/mL,3~5、7对α-葡萄糖苷酶的IC_(50)值为(1.54±0.15)~(10.53±0.38)μg/mL。结论化合物1~7、10为首次从菝葜属中分离得到,9、11为首次从该植物中分离得到。化合物8具有抗肿瘤活性,3~5、7具有α-葡萄糖苷酶抑制活性。AIM To study the chemical constituents from the water fraction of rhizoma of Smilax trinervula Miq.and their biological activities.METHODS Polyamide,silica gel,Sephadex LH-20,ODS and semi-preparative HPLC were used for isolation and purification,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The antitumor activities were determined by MTT mothod,and the inhibitory activities onα-glucosidase were determined by PNPG method.RESULTS Eleven compounds were isolated and identified as tyrosine(1),uridine(2),2-(2′,3′,4′-trihydroxybutyl)-6-(2″,3″,4″-trihydroxybutyl)-pyrazine(3),2-(1′,2′,3′,4′-tetrahydroxybutyl)-6-(2″,3″,4″-trihydroxybutyl)-pyrazine(4),2-(1′,2′,3′,4′-tetrahydroxybutyl)-5-(2″,3″,4″-trihydroxybutyl)-pyrazine(5),uracil(6),2-(1′,2′,3′,4′-tetrahydroxybutyl)-5-(1″,2″,3″,4″-tetrahydroxybutyl)-pyrazine(7),dioscin(8),shikimic acid(9),pyrazine(10),3,4-dihydroxyphenyethyl alcohol 8-O-β-D-glycopyranoside(11).The IC_(50)values of compounds 8 to human breast cancer cell MCF-7 was(2.36±0.26)μg/mL,and the IC_(50)values of compounds 3-5 and 7 toα-glucosidase were(1.54±0.15)-(10.53±0.38)μg/mL.CONCLUSION Compounds 1-7,10 are isolated from Smilax genus for the first time,and compound 9,11 are first isolated from this plant.Compound 8 has anti-tumor activity,and compounds 3-5,7 haveα-glucosidase inhibitory activities.

关 键 词：三脉菝葜 根茎 水部位 化学成分 分离鉴定 抗肿瘤活性 Α-葡萄糖苷酶 多羟基生物碱 

分 类 号：R284.1[医药卫生—中药学]