NaCl胁迫下嗜盐蒙地曲霉Aspergillus montevidensis ZYD4的转录组及代谢组分析  

Transcriptome and metabolome analysis of Aspergillus montevidensisZYD4 under NaCl stress

作　　者：张李旭 刘开辉 丁小维 李健苗 马雪妮 ZHANG Li-xu;LIU Kai-hui;DING Xiao-wei;LI Jian-miao;MA Xue-ni(School of Food Science and Engineering,Shaanxi University of Science&Technology,Xi′an 710021,China)

机构地区：[1]陕西科技大学食品科学与工程学院,陕西西安710021

出　　处：《陕西科技大学学报》2025年第2期64-72,共9页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金项目(31970120);陕西省科技厅社会发展科技计划项目(2023-YBSF-325)。

摘　　要：蒙地曲霉ZYD4是一株极端嗜盐真菌,可在饱和盐水中长期存活,是探究真核细胞嗜盐机制的重要微生物资源.利用NaCl诱导培养蒙地曲霉ZYD4菌株1 h,对诱导和未诱导的菌丝体进行透射电镜观察以及转录组高通量测序和非靶标代谢组分析.结果表明,盐胁迫下真菌细胞内自噬溶酶体(Autolysosome, ASS)的数量明显增加;与对照相比,盐诱导组共检测到1982个差异表达基因(Differentially Expressed Genes, DEGs);KEGG(Kyoto Encyclopedia of Genes and Genomes)分析发现,DEGs富集到MAPK(Mitogen-activated Protein Kinase)信号通路、细胞周期、核糖体、内吞作用和氨基糖等代谢途径;代谢组分析显示,差异代谢物有134个,主要包括谷氨酸、天冬氨酸、海藻糖、半乳糖、柠檬酸和棕榈酸等.本研究为探究嗜盐真菌耐盐胁迫及资源利用奠定基础.Aspergillus montevidensis ZYD4 is an extremely halophilic fungi that can survive in saturated salt water for a long time and is an important microbial resource for exploring the salt-tolerant mechanisms of eukaryotic cells.The Aspergillus montevidensis ZYD4 strain was induced and cultured with NaCl for 1 h,and the induced and uninduced mycelia were observed by transmission electron microscopy.Transcriptome and non-target metabolome of mycelia were also conducted under the same treatment.The results showed that the number of autolysosomes(Autolysosome,ASS)in fungi cells increased significantly under salt stress.Compared with the negative control,a total of 1982 differentially expressed genes(Differentially Expressed Genes,DEGs)were identified in the salt-induced group;KEGG(Kyoto Encyclopedia of Genes and Genomes)analysis found that DEGs were enriched in the MAPK(Mitogen-activated Protein Kinase)signaling pathway,cell cycle,ribosomes,endocytosis,amino sugar and other metabolic pathways.Metabolome analysis revealed that there were 134 differential metabolites,mainly including glutamic acid,aspartic acid,trehalose,galactose,citric acid and palmitic acid,etc.This study laid a foundation for exploring salt-tolerance mechanism and resource utilization of halophilic fungi.

关 键 词：嗜盐真菌 转录组 代谢组 

分 类 号：TS201.3[轻工技术与工程—食品科学] Q936[轻工技术与工程—食品科学与工程]