熟地黄提取物调控PI3K/AKT/mTOR信号通路增强鸡卵巢功能  

Rehmannia extract enhances ovarian functions of chicken via regulating PI3K/AKT/mTOR signaling pathway

作　　者：杨雅婷[1] 王晓童 刘兆梅 董杨勇 张毓萱 王冉[1] 潘驰宇 邱小燕[2] 肖雄[1] YANG Yating;WANG Xiaotong;LIU Zhaomei;DONG Yangyong;ZHANG Yuxuan;WANG Ran;PAN Chiyu;QIU Xiaoyan;XIAO Xiong(College of Veterinary Medicine,Southwest University,Rongchang,Chongqing 400715,China;College of Animal Science and Technology,Southwest University,Rongchang,Chongqing 400715,China)

机构地区：[1]西南大学动物医学院,重庆荣昌400715 [2]西南大学动物科学技术学院,重庆荣昌400715

出　　处：《中国兽医学报》2024年第12期2619-2625,2680,共8页Chinese Journal of Veterinary Science

基　　金：重庆市教委科学技术研究资助项目(KJZD-K202200208);重庆市研究生科研创新资助项目(CYS22246);中央高校基本科研业务费专项资金资助项目(XDJK2020B011,SWU-XDJH202307);大学生创新创业训练计划资助项目(S202210635390)。

摘　　要：为探究熟地黄提取物促进卵巢卵泡发育、缓解卵巢衰老及提高鸡生产性能的作用机制,本研究选用35周龄乌皮红冠产蛋鸡,随机分为空白对照组、0.1%、0.2%和0.4%熟地黄添加组进行饲喂试验;通过检测生殖激素及卵巢上相应受体,量化卵巢与卵泡内的细胞增殖与凋亡损伤,探寻熟地黄提升鸡生殖性能的组织学结构与分子调节的变化;基于PI3K/AKT/mTOR与AMPK信号通路,深入解析熟地黄促进卵巢卵泡发育、缓解卵巢衰老的作用机制,以丰富地方品种鸡的生殖调控理论,提升其生殖性能。结果显示,0.4%熟地黄提取物能够提高蛋鸡血清促卵泡生成素(FSH)、雌二醇(E_(2))和孕酮(P_(4))的含量,提升卵巢FSHR、ERβ和PCNA基因的mRNA相对表达量,增加PCNA蛋白含量,减少凋亡细胞数量,下调凋亡蛋白Cleaved-Caspase-3水平;熟地黄可增强鸡去甲肾上腺素的分泌,通过激活卵巢上α2A-AR以及下游PI3K/AKT/mTOR信号通路,从而有效减缓卵巢功能衰退,保持稳定高效的卵巢机能,且该功效并不依赖于AMPK信号通路。因此,0.4%熟地黄提取物可通过调控PI3K/AKT/mTOR信号通路而增强鸡的生殖功能。In order to explore the mechanism by which Rehmannia extract enhances ovarian follicle development,reduces ovarian aging,and improves the production performance of chickens,35-week-old black skin and red crest chickens were randomly divided into a blank control group and groups supplemented with 0.1%,0.2%and 0.4%Rehmannia extract for feeding experiments.Changes of histological structure and molecular regulation for improving the reproductive performance of black skin and red crest chicken were explored here by detecting the reproductive hormones and the corresponding receptors in ovary and quantifying the cell proliferation and apoptotic lesion.Based on the PI3K/AKT/mTOR and AMPK signaling pathways,mechanisms of Rehmannia extract on promoting ovarian follicle development and alleviating ovarian aging were further studied to enrich the reproductive regulation theory of native breed chickens and improve their reproductive performance.The results indicated that 0.4%Rehmannia extract could improve the levels of LH,E_(2)and P_(4)in serum,elevate the relative expression levels of FSHR,ERβand PCNA mRNA in ovarian tissues,increase the content of PCNA protein,and decrease the number of apoptosis cells and the level of apoptosis protein Cleaved-Caspase-3.By enhancing the secretion of norepinephrine and activating theα2A-AR and the downstream PI3K/AKT/mTOR signaling pathway in ovary,the Rehmannia extract could effectively relieve the decline of ovarian function and maintain stable and efficient ovarian functions,which was independent of AMPK signaling pathway.Therefore,0.4%Rehmanniaextract is conducive to enhancing the reproductive function viaregulating PI3K/AKT/mTOR signaling pathway.

关 键 词：熟地黄提取物 乌皮红冠鸡 卵巢 PI3K AKT MTOR 

分 类 号：S831.5[农业科学—畜牧学]