长链非编码RNA SNHG1对人胆囊癌细胞生物学行为的影响及机制  

Effect and mechanism of long non-coding RNA SNHG1 on biological behaviors of human gallbladder cancer cells

作　　者：田玉伟 王佳佳 刘红山 王要轩 李珂 薛焕洲 TIAN Yuwei;WANG Jiajia;LIU Hongshan;WANG Yaoxuan;LI Ke;XUE Huanzhou(Department of Hepatobiliary Pancreatic Surgery,Henan Provincial People's Hospital,Zhengzhou University People's Hospital,Zhengzhou,Henan 450003,China)

机构地区：[1]河南省人民医院肝胆胰腺外科,郑州大学人民医院,河南郑州450003

出　　处：《中华实用诊断与治疗杂志》2025年第1期21-26,共6页Journal of Chinese Practical Diagnosis and Therapy

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ20210063)。

摘　　要：目的 观察长链非编码RNA SNHG1在人胆囊癌细胞中表达的变化,探讨其对胆囊癌细胞增殖、凋亡的影响及机制。方法 取对数生长期人胆囊癌细胞株NOZC-1、人胆囊上皮细胞株HGBEC,采用实时荧光定量PCR法检测SNHG1相对表达量,采用荧光原位杂交实验检测SNHG1在NOZC-1细胞中的定位。将对数生长期NOZC-1细胞分为对照组(不作处理正常培养)、空载组(转染siRNA-NC)、干扰组(转染SNHG1-siRNA)。转染后培养48 h,3组采用CCK-8法检测细胞增殖率,采用流式细胞术检测细胞凋亡率,采用Western blot法检测核因子-κB(NF-κB) p65、NF-κB抑制蛋白(IκB)、p-IκB、Krüppel样因子5(KLF5)蛋白相对表达量,计算p-IκB/IκB。结果 NOZC-1细胞SNHG1相对表达量(3.776±0.274)高于HGBEC细胞(1.004±0.104)(t=2.191,P=0.013)。SNHG1在NOZC-1细胞中主要定位于细胞核。转染后培养48 h,3组细胞增殖率、凋亡率比较差异均有统计学意义(F=36.050,P<0.001;F=37.680,P<0.001)。干扰组细胞增殖率[(92.69±1.63)%]低于对照组[(100.00±1.84)%]和空载组[(98.12±1.71)%](P<0.05),细胞凋亡率[(4.51±0.11)%]高于对照组[(3.30±0.06)%]和空载组[(3.43±0.30)%](P<0.05),对照组细胞增殖率、细胞凋亡率与空载组比较差异均无统计学意义(P>0.05)。转染48 h,3组NF-κB p65、KLF5蛋白相对表达量及p-IκB/IκB比较差异均有统计学意义(F=17.60~649.70,P均<0.05)。干扰组细胞NF-κB p65(0.23±0.03)、KLF5(0.34±0.02)蛋白相对表达量及p-IκB/IκB(0.77±0.02)均低于对照组(0.49±0.01、0.88±0.01、1.00±0.10)和空载组(0.35±0.02、0.43±0.03、1.15±0.06)(P<0.05),对照组与空载组比较差异均无统计学意义(P>0.05)。结论 人胆囊癌细胞SNHG1表达增高,下调其表达可通过调控SNHG1/KLF5轴抑制NF-κB信号通路活性,抑制细胞增殖、促进细胞凋亡。Objective To observe the changes of long non-coding RNA SNHG1 in human gallbladder cancer cells,and to explore its impact and mechanism on the proliferation and apoptosis of gallbladder cancer cells.Methods Human gallbladder cancer cell line NOZC-1 and human gallbladder epithelial cell line HGBEC in logarithmic growth phase were selected,and the relative expression of SNHG1 was detected with real-time fluorescence quantitative PCR.The localization of SNHG1in NOZC-1cells was detected with fluorescence in situ hybridization.The NOZC-1cells in logarithmic growth phase were divided into the control group(normal culture without treatment),the empty vector group(transfected with siRNA-NC),and the interference group(transfected with SNHG1-siRNA).After 48-h culture in three groups,the cell proliferation rate was detected with CCK-8 method,the cell apoptosis rate was detected with flow cytometry,and the relative expressions of nuclear factor kappa-B(NF-κB)p65,inhibitor of NF-κB(IκB),and p-IκB and Krüppel-like factor 5(KLF5)proteins were detected with Western blot.The p-IκB/IκB ratio was calculated.Results The relative expression of SNHG1 was higher in NOZC-1cells(3.776±0.274)than that in HGBEC cells(1.004±0.104)(t=2.191,P=0.013).SNHG1 was mainly localized in the nucleus of NOZC-1cells.After 48-h culture,there were significant differences in the cell proliferation and apoptosis rates among three groups(F=36.050,P<0.001;F=37.680,P<0.001).The proliferation rate was lower in the interference group[(92.69±1.63)%]than that in the control group[(100.00±1.84)%]and the empty vector group [(98.12±1.71)%](P<0.05),and the apoptosis rate was higher in the interference group[(4.51±0.11)%]than that in the control group[(3.30±0.06)%]and the empty vector group[(3.43±0.30)%](P<0.05).There were no significant differences in the proliferation and apoptosis rates between the control group and the empty vector group(P>0.05).After 48-h transfection,the relative expressions of NF-κB p65and KLF5proteins,and p-IκB/IκB ratio showe

关 键 词：人胆囊癌细胞 NOZC-1 长链非编码RNA SNHG1 Krüppel样因子5 核因子-ΚB信号通路 

分 类 号：R735.8[医药卫生—肿瘤]