Chinese medicine Bupleuri Radix inhibits the proliferation of renal clear cell carcinoma is related to RORC  

作　　者：Yan Li Hao-Yu Wang An-Qi Lv Xun Xu Wei Yan Lang Guo Zhong-Min Zhang Bing-Qi Zhang Zheng-Ming Liao 

机构地区：[1]The First Clinical College of Hubei University of Traditional Chinese Medicine,Wuhan 430065,China [2]Department of Urology,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430061,China [3]Hubei Sizhen Laboratory,Wuhan 430061,China [4]Affiliated Hospital of Hubei University of Chinese Medicine,Wuhan 430061,China [5]College of Traditional Chinese Medicine,Hubei University of Traditional Chinese Medicine,Wuhan 430065,China

出　　处：《Integrative Medicine Discovery》2025年第5期1-12,共12页

基　　金：supported by Hubei Provincial Department of Science and Technology,Innovation and Development Joint Fund(No.2022CFD145);Young Talents Project of Hubei Provincial Administration of Traditional Chinese Medicine(No.ZY2023Q002).

摘　　要：Background:This research aims to investigate potential gene targets and mechanisms through which the traditional Chinese medicine(TCM)Bupleuri Radix(Chaihu)may impact on clear cell renal cell carcinoma(ccRCC)treatment.Methods:Public databases were employed to identify Cedrenol,an active component of Bupleuri Radix,and its associated gene targets.Among these,the gene RORC(also known as RORγ)was selected for detailed analysis due to its high expression correlation.Expression levels of RORC across various cancers were assessed using the TCGA pan-cancer dataset.Further analyses,including differential expression,prognostic relevance,immune infiltration,single-gene,and functional enrichment analyses,were conducted using the TCGA ccRCC dataset.Additionally,potential drug sensitivities and molecular docking interactions with RORC were explored using the GSCALite and CellMiner databases.The effects of RORC on ccRCC were also validated through cellular experiments.Results:RORC exhibited elevated expression in clear cell renal carcinoma tissue in contrast to normal tissues,and lower RORC expression was related to better prognosis.Immune cell infiltration analysis suggested that RORC may influence the penetration of cells that inhibit immune responses,such as regulatory T cells,thereby affecting ccRCC progression.Furthermore,molecular docking studies revealed that several drugs,including Axitinib,Docetaxel,Methotrexate,and Temsirolimus,have a high affinity for RORC and exhibit strong molecular binding.In cellular experiment experiments confirmed that RORC knockdown led to reduced proliferation,metastasis,and ccRCC cell invasion.Conclusion:The study implies that RORC may be a potential gene target for Bupleuri Radix in the management of ccRCC.

关 键 词：traditional Chinese medicine Bupleuri Radix(Chaihu) clear cell renal cell carcinoma urologic neoplasms RORC(RORγ) 

分 类 号：R73[医药卫生—肿瘤]