气相色谱-串联质谱法测定茶叶中的蒽醌残留量  

Determination of Anthraquinone Residue in Tea by Gas Chromatography-Tandem Mass Spectrometry

作　　者：黄旭 HUANG Xu(Liaoning Food Inspection and Testing Institute,Shenyang 110000,China)

机构地区：[1]辽宁省食品检验检测院,辽宁沈阳110000

出　　处：《食品安全导刊》2025年第9期81-83,共3页China Food Safety Magazine

摘　　要：目的:建立一种使用QuEChERS提取、分散固相萃取净化,气相色谱-串联质谱法测定茶叶中蒽醌残留量的方法。方法:样品加入同位素内标后,采用乙腈提取,加入盐包盐析分层,提取液经分散固相萃取净化,进一步稀释后进入气相色谱质谱联用仪进行分析。结果:蒽醌在0~200 ng·mL^(-1)浓度范围内呈现良好线性关系,线性相关系数大于0.995,定量限为0.01 mg·kg^(-1);3种不同茶叶样品基质在3个不同加标水平下的蒽醌回收率在90.2%~98.5%,相对标准偏差均小于3.0%。结论:该方法具有稳定、快速、重现性好等特点,能够满足茶叶中蒽醌残留分析的需求。Objective:To establish a method for the determination of anthraquinone residues in tea by QuEChERS extraction,dispersion solid phase extraction and gas chromatography-tandem mass spectrometry.Method:After adding isotope internal standard,the sample was extracted with acetonitrile,salt coated salting out and layering,and the extract was purified by dispersive solid phase extraction,further diluted,and then analyzed by gas chromatography-mass spectrometry.Result:Anthraquinone showed a good linear relationship in the concentration range of 0 ng·mL^(-1) to 200 ng·mL^(-1),the linear correlation coefficient was greater than 0.995,and the quantitative limit was 0.01 mg·kg^(-1).The recoveries of anthraquinone in three different tea sample matrices at three different spiked levels were between 90.2%and 98.5%,and the relative standard deviation was less than 3.0%.Conclusion:The method is stable,rapid and reproducible,and can meet the needs of anthraquinone residue analysis in tea.

关 键 词：茶叶 蒽醌 QUECHERS 气相色谱-串联质谱法 

分 类 号：R28[医药卫生—中药学]