超高效液相色谱-串联质谱法测定现制茶饮中11种荧光增白剂  

Determination of 11 fluorescent whitening agents in fresh tea drinks by ultra-high performance liquid chromatography-tandem mass spectrometry

作　　者：王春蕾 黄珍珍 李冰冰 戴丽 陈国清[1] 王坤[1] WANG Chunlei;HUANG Zhenzhen;LI Bingbing;DAI Li;CHEN Guoqing;WANG Kun(Center for Disease Control and Prevention of Yancheng,Yancheng Center of Jiangsu Food Safety Risk Monitoring,Yancheng Center of Molecular Biology in Public Health,Jiangsu Yancheng 224000,China)

机构地区：[1]盐城市疾病预防控制中心,江苏省食品安全风险监测重点实验室,盐城市公共卫生分子生物学重点实验室,江苏盐城224000

出　　处：《中国食品卫生杂志》2024年第12期1333-1341,共9页Chinese Journal of Food Hygiene

基　　金：盐城市公共卫生分子生物学重点实验室(盐卫规划[2023]19号);江苏省食品安全风险监测重点实验室(苏卫疾控[2023]6号)。

摘　　要：目的建立超高效液相色谱-串联质谱法测定现制茶饮中11种荧光增白剂的方法。方法样品经N,N-二甲基甲酰胺/水/三乙胺(70∶29∶1,V/V/V)超声提取,加适量沉淀剂,离心过滤后,进UPLC-MS/MS系统,以乙腈-0.2%氨水为流动相,梯度洗脱,经Waters BEH C_(18)(2.1 mm×100 mm,1.7μm)色谱柱分离,采用ESI源,正负离子同时扫描的多反应监测模式进行检测,外标法定量。结果11种荧光增白剂在各自的线性范围内线性良好,相关系数(r)均>0.995,方法检出限为0.003~0.03 mg/kg,方法定量限0.01~0.1 mg/kg,在奶茶、果茶和咖啡3类现制茶饮中进行低、中、高3水平加标,回收率范围为71.7%~116.9%,相对标准偏差(RSDs)范围为0.7%~9.9%。结论该方法简单、高效、准确,适用于奶茶、果茶和咖啡等现制茶饮中11种荧光增白剂的检测,为现制茶饮相关的食品安全监管提供方法。Objective To establish an analytical method for determination of 11 fluorescent whitening agents in fresh tea drinks using ultra-high performance liquid chromatography-tandem mass spectrometry.Methods The samples were extracted by sonication of N,N-dimethylformamide/water/triethylamine(70∶29∶1,V/V/V),added with appropriate precipitant,centrifuged and filtered,finally injected into the UPLC-MS/MS system.The samples were separated on a Waters BEH C_(18)column(2.1 mm×50 mm,1.7μm)with acetonitrile and 0.2%ammonia as mobile phase,and detected using electrospray ionization in multiple reaction monitoring mode via positive and negative ions scanning.Compounds were quantified by external standard method.Results The 11 fluorescent whitening agents had good linearity within the measured concentration range,and the correlation coefficients(r)were all above 0.995.The limits of detection were 0.003-0.03 mg/kg.The limits of quantitation were 0.01-0.1 mg/kg.The recoveries were 71.7%-116.%and the relative standard deviations were 0.7%-9.9%in three kinds of fresh tea drinks including milk tea,fruit tea and coffee.Conclusion The proposed method is simple,highly efficient,accurate,and suitable for the detection of 11 kinds of fluorescent whitening agents in fresh tea drinks such as milk tea,fruit tea and coffee,which provides a methods for food safety supervision related to fresh tea production.

关 键 词：现制茶饮 荧光增白剂 超高效液相色谱-串联质谱 

分 类 号：R155[医药卫生—营养与食品卫生学]