基于表面增强拉曼散射的深层组织肿瘤活体成像技术的研究进展  

Recent Advances in SERS(Surface-Enhanced Raman Scattering)for Deep-Tissue In Vivo Cancer Imaging

作　　者：吴红 肖莘芹 胡羽君 杨江 WU Hong;XIAO Xinqin;HU Yujun;YANG Jiang(State Key Laboratory of Oncology in South China,Guangdong Provincial Clinical Research Center for Cancer,Sun Yat-sen University Cancer Center,Guangzhou 510060,China)

机构地区：[1]华南恶性肿瘤防治全国重点实验室,广东省恶性肿瘤临床医学研究中心,中山大学肿瘤防治中心,广州510060

出　　处：《中国细胞生物学学报》2025年第3期433-449,共17页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:52271196、82472041);中山大学高校基本科研业务费(批准号:31610026);中山大学肿瘤防治中心青年人才提升计划(批准号:YTP-SYSUCC-0024)资助的课题。

摘　　要：表面增强拉曼散射(surface-enhanced Raman scattering,SERS)作为一种高灵敏度、高分辨率的光谱分析技术,在生物医学领域,特别是癌症的早期检测和非侵入性的活体成像中展现出了巨大的临床应用潜力。生物组织中的光散射、自发荧光和光吸收效应的增强,显著影响了光学成像技术在深层组织中的成像效果,进而导致了误诊和不完全的手术切除。随着分子影像的快速发展,SERS探针因其高灵敏度、分子靶向性和多重检测的独特优势,受到广泛关注。近年来,SERS结合透射拉曼、近红外二区拉曼及空间偏移拉曼等具有组织穿透深度的技术获得了重要进展,为非侵入性肿瘤活体成像提供了新思路和解决方案。该文回顾了基于SERS的深层组织活体成像的最新研究进展,讨论了其在临床转化、多模态及诊疗一体化中的应用前景,并展望了未来的发展方向,旨在促进其与临床医学的交叉融合,为癌症的早期诊断、手术导航和个体化治疗开辟新的路径。SERS(surface-enhanced Raman scattering),as a highly sensitive and high-resolution spectroscopic analytical technique,has demonstrated tremendous clinical application potential in biomedicine,particularly in early cancer detection and non-invasive in vivo imaging.However,the effects of light scattering,autofluorescence,and absorption in biological tissues significantly impair the performance of optical imaging techniques in deep tissues,leading to misdiagnosis and incomplete surgical resection.With rapid advances in molecular imaging,SERS probes have attracted widespread attention due to their unique advantages of high sensitivity,molecular targeting,and multiplexing capabilities.In recent years,the combination of SERS with emerging specialized Raman techniques such as transmission Raman,near-infrared-II Raman,and SORS(spatially offset Raman scattering)has achieved significant progress in cancer imaging.Such advancements provide new insights and feasible solutions for non-invasively imaging tumors buried deep in tissues.This review comprehensively overviews the latest developments in SERS cancer imaging in living subjects and discusses their application prospects in clinical translation,multimodal imaging,and theranostics.Furthermore,future research directions are envisaged to focus on interdisciplinary and clinical investigations.The aim is to pave new paths for early cancer diagnosis,surgical navigation,and personalized therapy,ultimately improving patient outcomes.

关 键 词：深层组织成像 肿瘤活体成像 表面增强拉曼散射 近红外二区拉曼光谱 空间偏移拉曼光谱 临床转化 

分 类 号：R73[医药卫生—肿瘤]