纳米银-聚己内酯-明胶纤维膜的制备及其对人脑胶质瘤的抗增殖作用初探  

Preparation of AgNPs-PCL-Gel fiber membrane and preliminary exploration of its anti-proliferative effect on glioma

作　　者：史梦柔 李锡晶[1] 程琳[1] 程鹏[1] 赵秀梅[1] 宗文辉 SHI Mengrou;LI Xijing;CHENG Lin;CHENG Peng;ZHAO Xiumei;ZONG Wenhui(Tianjin Institute of Medical and Pharmaceutical Science,Tianjin 300020,China)

机构地区：[1]天津市医药科学研究所,天津300020

出　　处：《药学研究》2025年第3期231-240,共10页Journal of Pharmaceutical Research

基　　金：天津市应用基础研究多元投入基金项目(No.21JCYBJC01680);天津市卫生健康科技项目(No.TJWJ2021QN076)。

摘　　要：目的筛选制备纳米银-聚己内酯-明胶(AgNPs-PCL-Gel)纳米纤维膜的最佳条件并初步探究其抑制人脑胶质瘤增殖的效果。方法通过静电纺丝技术将姜黄素(Cur)负载到PCL和Gel共纺的纳米纤维膜中,随后将Cur-PCL-Gel纳米纤维膜置于硝酸银(AgNO_(3))水溶液中,制备AgNPs-PCL-Gel纳米纤维膜。采用正交实验优化了静电纺丝条件,利用扫描电子显微镜观察纤维和AgNPs形态,能谱仪分析AgNPs的含量,并对材料的溶胀性能、亲水性能、降解性能、体外释放性能及体外抗人脑胶质瘤U-251MG细胞增殖作用进行评价。结果在最优工艺PCL∶Gel=5∶5,负载电压16 kV、推液速度0.0008 mm·s^(-1)、接收距离15 cm条件下可成功制备AgNPs-PCL-Gel纳米纤维。合成的AgNPs存在纳米纤维上,呈球形,粒径小于30 nm。反应96 h,生成的AgNPs数量达到峰值。纳米纤维膜溶胀性能和亲水性能良好,其在pH 6.2的人工脑脊液中降解速率较pH 7.4更快。体外释放研究也表明,其在pH 6.2的释放介质中释药更快,更完全。AgNPs-PCL-Gel纳米纤维膜对U-251MG细胞有明显的细胞毒性作用,IC_(50)值为0.15 mg·mL^(-1)。结论AgNPs在纳米纤维上分布均匀,未发现团聚现象,调控反应时间可控制AgNPs的生成量。纤维中AgNPs含量越高,纤维膜越亲水。纳米纤维膜释药性能良好,具有缓释作用。AgNPs-PCL-Gel纳米纤维膜具有良好的抑制U-251MG细胞增殖的作用,在抗脑胶质瘤领域表现出应用潜力。Objective To screen the optimal conditions for preparing AgNPs-PCL-Gel nanofiber membrane and explore its inhibitory effect on the proliferation of glioma.Methods Cur was loaded onto PCL and Gel co spun nanofiber membranes using electrospinning technology,and then the Cur-PCL-Gel nanofiber membrane was placed in an aqueous solution of silver nitrate(AgNO 3)to prepare AgNPs-PCL-Gel nanofiber membranes.Orthogonal optimization of electrospinning conditions was carried out,and the morphology of fibers and AgNPs was observed by scanning electron microscopy.The content of AgNPs was analyzed by energy dispersive spectroscopy,and the swelling performance,hydrophilicity,degradation performance,in vitro release performance,and in vitro anti glioma U-251MG cell proliferation effect of the material were evaluated.Results AgNPs-PCL-Gel nanofibers were prepared under the optimal conditions of PCL∶Gel=5∶5,load voltage of 16 kV,liquid pushing speed of 0.0008 mm·s^(-1),and receiving distance of 15 cm.The synthesized AgNPs exist on the nanofibers in a spherical shape with a particle size of less than 30 nm.After 96 hours of reaction,the generated AgNPs reached their peak.The nanofiber membrane has good swelling and hydrophilic properties,and its degradation rate in artificial cerebrospinal fluid at pH 6.2 is faster than that at pH 7.4.In vitro release studies also show that it releases drugs faster and more completely in the release medium at pH 6.2.AgNPs-PCL-Gel nanofiber membrane has a significant cytotoxic effect on U-251MG cells,with an IC_(50) value of 0.15 mg·mL^(-1).Conclusion AgNPs are evenly distributed on the nanofibers,and no agglomeration phenomenon was observed.Regulating the reaction time can control the generation of AgNPs.The higher the content of AgNPs in the fiber,the more hydrophilic the fiber membrane becomes.The nanofiber membrane has good drug release performance and a sustained release effect.AgNPs-PCL-Gel nanofiber membrane has a good inhibitory effect on the proliferation of U-251MG cells and shows p

关 键 词：纳米银 姜黄素 纳米纤维膜 静电纺丝 脑胶质瘤 

分 类 号：R739.41[医药卫生—肿瘤]