大肠癌湿热证患者舌苔代谢组学分析  

Metabolomic analysis of tongue coating in colorectal cancer patients with damp-heat syndrome

作　　者：黄正阳 陈健慧 冯丹青[1] 朱惠蓉[1] 程悦蕾[1] HUANG Zhengyang;CHEN Jianhui;FENG Danqing;ZHU Huirong;CHENG Yuelei(Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200021,China)

机构地区：[1]上海中医药大学附属曙光医院,上海200021

出　　处：《上海中医药杂志》2025年第4期17-24,共8页Shanghai Journal of Traditional Chinese Medicine

基　　金：上海市卫健委科研项目(202340156);上海中医药大学科研项目(2016YSN48)。

摘　　要：目的借助代谢组学技术分析大肠癌湿热证患者舌苔的生物学特性。方法采集大肠癌湿热证、大肠癌非湿热证患者及健康人群的舌苔标本,分别设为湿热证组、非湿热证组、健康人群组,采用液相色谱-质谱技术检测各样本的代谢成分,获得代谢指纹图谱后进行主成分分析、偏最小二乘法判别分析、正交偏最小二乘法判别分析,得到舌苔的相关代谢产物,寻找相应的代谢通路。结果大肠癌湿热证患者舌苔的差异代谢化合物主要为激素和传递介质、核酸、肽类等,主要的差异代谢产物有乙酰胆碱、胞嘧啶、L-色氨酸、5-羟色胺等,差异代谢通路主要集中于脂类代谢、氨基酸代谢、核酸代谢、消化系统、神经系统、感觉系统等。结论大肠癌湿热证患者舌苔具有一定的代谢组学特征,中医舌诊与现代技术相结合是继承和发扬中医特色的重要手段,可以为进一步探索大肠癌的形成和发展提供参考,从而指导治疗。Objective To investigate the biological characteristics of tongue coating in colorectal cancer patients with damp-heat syndrome using metabolomics technology.Methods Tongue coating samples were collected from colorectal cancer patients with damp-heat syndrome,patients with non-damp-heat syndrome,and healthy controls.These groups were designated as the damp-heat syndrome group,non-damp-heat syndrome group,and healthy control group,respectively.Liquid chromatography-mass spectrometry(LC-MS)was used to detect the metabolites in each sample.After obtaining the metabolic fingerprinting,principal component analysis(PCA),partial least squares discriminant analysis(PLS-DA),and orthogonal partial least squares discriminant analysis(OPLS-DA)were performed to identify the related metabolites and explore the associated metabolic pathways.Results The differential metabolites in the tongue coating of colorectal cancer patients with damp-heat syndrome were primarily composed of hormones,signaling mediators,nucleic acids,and peptides,etc.Key differential metabolites included acetylcholine,cytosine,L-tryptophan,and serotonin.The major differential metabolic pathways involved lipid metabolism,amino acid metabolism,nucleic acid metabolism,the digestive system,the nervous system,and sensory systems.Conclusions The tongue coating in colorectal cancer patients with damp-heat syndrome exhibits distinct metabolomic characteristics.The integration of traditional Chinese medicine(TCM)tongue diagnosis with modern analytical technologies is an important method for inheriting and promoting the distinctive features of TCM.These findings offer insights for further investigation into the pathogenesis and progression of colorectal cancer,thereby guiding treatment strategies.

关 键 词：大肠癌 结直肠癌 湿热证 舌苔 代谢组学 

分 类 号：R73[医药卫生—肿瘤]